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Proteomic Analysis Uncovers Measles Virus Protein C Interaction With p65–iASPP Protein Complex

Abstract : Viruses manipulate the central machineries of host cells to their advantage. They prevent host cell antiviral responses to create a favorable environment for their survival and propagation. Measles virus (MV) encodes two nonstructural proteins MV-V and MV-C known to counteract the host interferon response and to regulate cell death pathways. Several molecular mechanisms underlining MV-V regulation of innate immunity and cell death pathways have been proposed, whereas MV-C host-interacting proteins are less studied. We suggest that some cellular factors that are controlled by MV-C protein during viral replication could be components of innate immunity and the cell death pathways. To determine which host factors are targeted by MV-C, we captured both direct and indirect host-interacting proteins of MV-C protein. For this, we used a strategy based on recombinant viruses expressing tagged viral proteins followed by affinity purification and a bottom-up mass spectrometry analysis. From the list of host proteins specifically interacting with MV-C protein in different cell lines, we selected the host targets that belong to immunity and cell death pathways for further validation. Direct protein interaction partners of MV-C were determined by applying protein complementation assay and the bioluminescence resonance energy transfer approach. As a result, we found that MV-C protein specifically interacts with p65-iASPP protein complex that controls both cell death and innate immunity pathways and evaluated the significance of these host factors on virus replication.
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Contributor : Mariette Matondo Connect in order to contact the contributor
Submitted on : Monday, March 29, 2021 - 11:50:33 AM
Last modification on : Friday, August 5, 2022 - 12:02:33 PM
Long-term archiving on: : Wednesday, June 30, 2021 - 6:25:11 PM


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Alice Meignié, Chantal Combredet, Marc Santolini, István Kovács, Thibaut Douché, et al.. Proteomic Analysis Uncovers Measles Virus Protein C Interaction With p65–iASPP Protein Complex. Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2021, 20, pp.100049. ⟨10.1016/j.mcpro.2021.100049⟩. ⟨pasteur-03184181⟩



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