Lipopolysaccharides from six strains of Acetobacter diazotrophicus

Thierry Fontaine (1) , Marilia Penteado Stephan (2) , Laurent Debarbieux (3) , José Osvaldo Previato (1) , Lucia Mendonça-Préviato (1)

1 UFRJ - Universidade Federal do Rio de Janeiro
2 Embrapa Solos
3 Laboratoire de chimie biologique [Lille 1]

Lipopolysaccharides from six nitrogen-fixing strains of Acetobacter diazotrophicus (PR2, PAW, PALS, PR4, PR14, PR20), isolated from sugarcane, were purified by phenol-water extraction and ultracentrifugation. The relatively large molecular mass observed by SDS-PAGE indicated that the lipopolysaccharides of each strain possessed an O-side chain. Analysis of each lipopolysaccharide by calorimetric assays and by gas liquid chromatography/mass spectrometry combination showed that the core and lipid A composition was similar for all strains, containing 3-deoxy-D-manno-2-octulosonic acid, glucosamine and fatty acid (16-0, 3-OH-14, 2-OH-16:0, 3-OH-16:O). The neutral sugar composition showed the predominance of 6-deoxy-hexose (rhamnose and fucose) and ribose, in comparison with hexose (glucose, galactose, mannose). The presence of 6-deoxy-hexose and ribose containing O-side chains is discussed as a way of discriminating A. diazotrophicus from other Acetobacter species.

Keywords: Acetobacter diazotrophicus; Lipopolysaccharide; Sugarcane; 6-Deoxy-hexose; Ribose

Domains: Life Sciences [q-bio]

Thierry Fontaine, Marilia Penteado Stephan, Laurent Debarbieux, José Osvaldo Previato, Lucia Mendonça-Préviato. Lipopolysaccharides from six strains of Acetobacter diazotrophicus. FEMS Microbiology Letters, 1995, 132 (1-2), pp.45-50. ⟨10.1111/j.1574-6968.1995.tb07808.x⟩. ⟨pasteur-03165709⟩