In Vivo Bacteriophage Biodistribution

Nicolas Dufour (1, 2, 3) , Raphaëlle Delattre (1, 3, 4) , Laurent Debarbieux (1)

1 BMGE - Biologie Moléculaire du Gène chez les Extrêmophiles
2 Centre Hospitalier René Dubos [Pontoise]
3 IAME (UMR_S_1137 / U1137) - Infection, Anti-microbiens, Modélisation, Evolution
4 Hôpital Beaujon

At the dawn of the renaissance of bacteriophage therapy, the full acceptation of bacteriophages as anti-bacterial agents requires the determination of their basic pharmacokinetic parameters. Such data, known for all conventional drugs used in human and veterinary medicine, allow optimizing dose regimens, efficacy, and help to limit toxicity. Here, we describe basic methods to experimentally obtain pharmacokinetic data and give also examples of data calculation to determine key parameters related to the biodistribution and elimination of bacteriophages in vivo.

Mots clés: Bacteriophage; Bacteriophage therapy; Clearance; Half-life; Pharmacokinetic; Volume of distribution

Domaines: Sciences du Vivant [q-bio]

Nicolas Dufour, Raphaëlle Delattre, Laurent Debarbieux. In Vivo Bacteriophage Biodistribution. Joana AZEREDO, Sanna SILLANKORVA. Bacteriophage Therapy. From Lab to Clinical Practice, 1683, Springer Protocols, pp.123-137, 2018, Methods in Molecular Biology, 978-1-4939-7395-8. ⟨10.1007/978-1-4939-7395-8_11⟩. ⟨pasteur-03164482⟩