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Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life

Frédéric Rimet 1, * Eva Aylagas 2 Ángel Borja 3, 4 Agnes Bouchez 1 Alexis Canino 1 Christian Chauvin 5 Teofana Chonova 1 Fedor Ciampor Jr 6 Filipe Costa 7 Benoit Ferrari 8 Romain Gastineau 9 Chloé Goulon 1 Muriel Gugger 10 Maria Holzmann 11 Regine Jahn 12 Maria Kahlert 13 Wolf-Henning Kusber 12 Christophe Laplace-Treyture 5 Florian Leese 14 Frederik Leliaert 15 David Mann 16, 17 Frédéric Marchand 18 Vona Méléder 19 Jan Pawlowski 11, 20, 21 Serena Rasconi 1 Sinziana Rivera 1 Rodolphe Rougerie 22 Magali Schweizer 23 Rosa Trobajo 16 Valentin Vasselon 24 Régis Vivien 8 Alexander Weigand 25 Andrzej Witkowski 9 Jonas Zimmermann 12 Torbjørn Ekrem 26
Abstract : DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quality and traceability of the metadata associated with the reference barcodes used for identification. Sufficient metadata, as well as vouchers, corresponding to each reference barcode must be available to ensure reliable barcode library curation and, thereby, provide trustworthy baselines for downstream molecular species identification. This document (1) specifies the data and metadata required to ensure the relevance, the accessibility and traceability of DNA barcodes and (2) specifies the recommendations for DNA harvesting and for the storage of both voucher specimens/samples and barcode data.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03159791
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Submitted on : Thursday, March 4, 2021 - 5:01:51 PM
Last modification on : Tuesday, September 21, 2021 - 4:06:05 PM
Long-term archiving on: : Saturday, June 5, 2021 - 7:16:12 PM

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Frédéric Rimet, Eva Aylagas, Ángel Borja, Agnes Bouchez, Alexis Canino, et al.. Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life. Metabarcoding and Metagenomics, Pensoft Publishers 2021, 5, pp.e58056. ⟨10.3897/mbmg.5.58056⟩. ⟨pasteur-03159791⟩

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