Skip to Main content Skip to Navigation
New interface
Journal articles

E-cadherin focuses protrusion formation at the front of migrating cells by impeding actin flow

Abstract : The migration of many cell types relies on the formation of actomyosin-dependent protrusions called blebs, but the mechanisms responsible for focusing this kind of protrusive activity to the cell front are largely unknown. Here, we employ zebrafish primordial germ cells (PGCs) as a model to study the role of cell-cell adhesion in bleb-driven single-cell migration in vivo. Utilizing a range of genetic, reverse genetic and mathematical tools, we define a previously unknown role for E-cadherin in confining bleb-type protrusions to the leading edge of the cell. We show that E-cadherin-mediated frictional forces impede the backwards flow of actomyosin-rich structures that define the domain where protrusions are preferentially generated. In this way, E-cadherin confines the bleb-forming region to a restricted area at the cell front and reinforces the front-rear axis of migrating cells. Accordingly, when E-cadherin activity is reduced, the bleb-forming area expands, thus compromising the directional persistence of the cells.
Document type :
Journal articles
Complete list of metadata
Contributor : Aleix Boquet-Pujadas Connect in order to contact the contributor
Submitted on : Thursday, February 25, 2021 - 9:55:32 PM
Last modification on : Thursday, April 7, 2022 - 10:10:44 AM
Long-term archiving on: : Wednesday, May 26, 2021 - 7:27:49 PM


Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License




Cecilia Grimaldi, Isabel Schumacher, Aleix Boquet-Pujadas, Katsiaryna Tarbashevich, Bart Eduard Vos, et al.. E-cadherin focuses protrusion formation at the front of migrating cells by impeding actin flow. Nature Communications, 2020, 11 (1), pp.5397. ⟨10.1038/s41467-020-19114-z⟩. ⟨pasteur-03152922⟩



Record views


Files downloads