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Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae O1 in complex with antigen: Molecular basis for serotype specificity

Abstract : The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex with synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The upstream terminal O-SP monosaccharide is shown to be the primary antigenic determinant. Additional perosamine residues protrude outwards from the Ab surface and contribute only marginally to the binding affinity and specificity. A complementary water-excluding hydrophobic interface and five Ab-Ag hydrogen bonds are crucial for carbohydrate recognition. The structure reported here explains the serotype specificity of anti-Ogawa Abs and provides a rational basis toward the development of a synthetic carbohydrate-based anti-cholera vaccine.
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Submitted on : Wednesday, February 17, 2021 - 6:56:45 PM
Last modification on : Thursday, March 25, 2021 - 3:20:05 AM

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S. Villeneuve, H. Souchon, M.-M. Riottot, J.-C. Mazie, P.-S. Lei, et al.. Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae O1 in complex with antigen: Molecular basis for serotype specificity. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2000, 97 (15), pp.8433-8438. ⟨10.1073/pnas.060022997⟩. ⟨pasteur-03144732⟩

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