Physical estimation of triplet phases from two new proteins

F. Mo (1) , R. Mathiesen (1) , P.M. Alzari (2) , J. Lescar (3) , B. Rasmussen (4)

1 Institutt for Fysikk [Trondheim]
2 Biochimie Structurale
3 ESRF - European Synchroton Radiation Facility [Grenoble]
4 EMBL - European Molecular Biology Laboratory [Grenoble]

Three-beam interference experiments have been performed with crystals of two glycosidases: guinea-fowl hexagonal lysozyme, MW 14.3 kDa, and C. thermocellum endoglucanase CelA, MW 40 kDa. In both cases triplet phases could be estimated. Experimental parameters and details of the procedure are presented along with some examples of the results. The average differences between the estimated phases and those calculated from the crystallographic refinements were 17.9 and 15.9 degrees, respectively. A brief discussion of alternative methods for physical phase acquisition is given, including possible strategies for the measurement and application of experimental phases in macromolecular crystallography.

Keywords: triplet phases

Domains: Chemical Sciences; Cristallography; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Structural Biology [q-bio.BM]; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Computer Science [cs]; Bioinformatics [q-bio.QM]; Physics [physics]; Physics [physics]; Biological Physics [physics.bio-ph]; Life Sciences [q-bio]; Cellular Biology

F. Mo, R. Mathiesen, P.M. Alzari, J. Lescar, B. Rasmussen. Physical estimation of triplet phases from two new proteins. Acta crystallographica Section D : Structural biology [1993-..], 2002, 58 (10), pp.1780-1786. ⟨10.1107/s0907444902012222⟩. ⟨pasteur-03144654⟩