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Journal articles
Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens
M. Fogg
1
P. Alzari
2
M. Bahar
3
I. Bertini
4
J.-M. Betton
1
W. Burmeister
5
C. Cambillau
6
B. Canard
6
M. Carrondo
7
M. Coll
8
S. Daenke
3
O. Dym
9
M.-P. Egloff
6
F. Enguita
7
A. Geerlof
10
A. Haouz
2
T. Jones
11
Q. Ma
10
S. Manicka
9
M. Migliardi
4
P. Nordlund
12
R. Owens
3
Y. Peleg
9
G. Schneider
13
R. Schnell
13
D. Stuart
3
N. Tarbouriech
5
T. Unge
11
A. Wilkinson
1
M. Wilmanns
10
K. Wilson
1
O. Zimhony
9
J. Grimes
3, *
*
Corresponding author
J.-M. Betton 1
Author
W. P. Burmeister 5
Author
C. Cambillau 6
Author
B. Canard 6
Author IdHAL : bruno-canard ORCID : https://orcid.org/0000-0003-4924-1991
M.-P. Egloff 6
Author
F. J. Enguita 7
Author
A. Geerlof 10
Author
A. Haouz 2
Author IdHAL : ahmed-haouz ORCID : https://orcid.org/0000-0003-1196-1635
J. M. Grimes 3
Correspondent author
1
University of York [York, UK] (Heslington, York, YO10 5DD - United Kingdom)
2
Biochimie Structurale (28 Rue du Docteur Roux 75724 Paris Cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : URA2185 (France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
3
The Wellcome Trust Centre for Human Genetics [Oxford] (Roosevelt Drive, Oxford OX3 7BN, UK - United Kingdom)
- University of Oxford [Oxford] (Wellington Square, Oxford OX1 2JD - United Kingdom)
4
CIRMMP - Consorzio Interuniversitario Risonanze Magnetiche di Metallo Proteine (Via Sacconi, 6, 50019 Sesto Fiorentino (FI) - Italy)
- UNISI - Università degli Studi di Siena = University of Siena (Rettorato - Via Banchi di Sotto 55 - 53100 Siena - Italy)
- UNIFI - Università degli Studi di Firenze = University of Florence [Firenze] (Piazza di San Marco, 4, 50121 Firenze FI, Italie - Italy)
- University of Bologna (Via Zamboni, 33 - 40126 Bologna - Italy)
- Partenaires INRAE (Cette structure regroupe l'ensemble des structures créées pour la reprise des données ProdInra - France)
5
EMBL - European Molecular Biology Laboratory [Grenoble] (71 avenue des Martyrs
CS 90181
38042 Grenoble Cedex 9 - France)
6
AFMB - Architecture et fonction des Macromolécules Biologiques - UMR 6098 (Marseille - France)
- Université de Provence - Aix-Marseille 1 (3, place Victor Hugo - 13331 Marseille Cedex 03 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR6098 (France)
7
ITQB - Instituto de Tecnologia Química e Biológica António Xavier (Avenida da República
Estação Agronómica Nacional
2780-157 Oeiras - Portugal)
- NOVA - Universidade Nova de Lisboa = NOVA University Lisbon (Campus de Campolide 1099-085 Lisboa - Portugal)
8
Institut de Biologia Molecular de Barcelona (C/ Baldiri Reixac, 4-8, Torre R, 3era Planta, 08028 Barcelona - Spain)
- CSIC - Consejo Superior de Investigaciones Científicas [Madrid] (Spanish National Research Council Serrano 117, 28006 Madrid - Spain)
9
Weizmann Institute of Science [Rehovot, Israël] (234 Herzl Street, Rehovot 7610001 - Israel)
10
EMBL - European Molecular Biology Laboratory [Hamburg] (c/o DESY, Building 25A, Notkestraße 85, 22607 Hamburg - Germany)
11
BMC - Biomedical Center = Biomedicinskt centrum [Uppsala, Sueden] (Uppsala SE-751 24 - Sweden)
- Uppsala University (Box 256, SE-751 05 Uppsala - Sweden)
12
Stockholm University (Stockholm University, SE-106 91 Stockholm, Sweden - Sweden)
13
Karolinska Institutet [Stockholm] (Solnavägen 1, 171 77 Solna
Stockholm - Sweden)
Abstract : The Structural Proteomics In Europe (SPINE) programme is aimed at the development and implementation of high-throughput technologies for the efficient structure determination of proteins of biomedical importance, such as those of bacterial and viral pathogens linked to human health. Despite the challenging nature of some of these targets, 175 novel pathogen protein structures (approximately 220 including complexes) have been determined to date. Here the impact of several technologies on the structural determination of proteins from human pathogens is illustrated with selected examples, including the parallel expression of multiple constructs, the use of standardized refolding protocols and optimized crystallization screens.
Document type :
Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03139239
Contributor : Marie de Tarragon Connect in order to contact the contributor
Submitted on : Thursday, February 11, 2021 - 6:06:12 PM
Last modification on : Monday, January 10, 2022 - 11:44:05 AM
Contributor : Marie de Tarragon Connect in order to contact the contributor
Submitted on : Thursday, February 11, 2021 - 6:06:12 PM
Last modification on : Monday, January 10, 2022 - 11:44:05 AM
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Identifiers
- HAL Id : pasteur-03139239, version 1
- PUBMED : 17001096
- DOI : 10.1107/S0907444906030915
- PUBMEDCENTRAL : PMC7161641
Citation
M. Fogg, P. Alzari, M. Bahar, I. Bertini, J.-M. Betton, et al.. Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens. Acta Crystallographica Section D: Biological Crystallography, International Union of Crystallography, 2006, 62 (10), pp.1196-1207. ⟨10.1107/S0907444906030915⟩. ⟨pasteur-03139239⟩
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