Evolution of abiotic cubane chemistries in a nucleic acid aptamer allows selective recognition of a malaria biomarker

Yee-Wai Cheung (1) , Pascal Röthlisberger (2) , Ariel E Mechaly (3) , Patrick Weber (3) , Fabienne Levi-Acobas (2) , Young Lo (1) , Alvin W C Wong (1) , Andrew B Kinghorn (1) , Ahmed Haouz (3) , G Paul Savage (4) , Marcel Hollenstein (2) , Julian A Tanner (1)

1 HKU - The University of Hong Kong
2 Chimie bioorganique des acides nucléiques - Bioorganic chemistry of nucleic acids
3 Cristallographie (Plateforme) - Crystallography (Platform)
4 CSIRO - Division of Manufacturing Science and Technology

for the overall study design and data interpretation. Y.W.C. performed all cubamer selections, characterized the cubamers and oversaw all application experiments. A.W.C.W., Y.L., and A.B.K. were involved in aspects of cubamer characterization and in assay design. F.L.-A. expressed and purified the proteins and performed the biochemical characterization of the triphosphate. P.R. performed all chemical syntheses and was involved in aspects of experimental design of cubamer structural analysis. P.R. was also significantly involved in all data interpretation. G.P.S. was involved in steps of synthesis relating to cubanes and advised on study design.

Keywords: Modified aptamer; SELEX; X-ray crystallography; cubane; malaria diagnosis Author Contributions J.A.T.; M.H.; Y.W.C. and P.R. were responsible; malaria diagnosis; modified aptamer

Domains: Chemical Sciences; Chemical Sciences; Organic chemistry

Yee-Wai Cheung, Pascal Röthlisberger, Ariel E Mechaly, Patrick Weber, Fabienne Levi-Acobas, et al.. Evolution of abiotic cubane chemistries in a nucleic acid aptamer allows selective recognition of a malaria biomarker. Proceedings of the National Academy of Sciences of the United States of America, 2020, 117 (29), pp.16790-16798. ⟨10.1073/pnas.2003267117⟩. ⟨pasteur-03138380⟩