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Molecular Cloning of a Monoclonal Anti-Tumor Antibody Specific for the Tn Antigen and Expression of an Active Single-Chain Fv Fragment

Abstract : We report here the first amino acid sequence of an anti-Tn monoclonal antibody raised against human breast cancer cells and show that a single chain Fv fragment of this IgM retains the Tn-binding specificity as defined by functional assays with asialo-OSM and membrane extracts from MCF-7 cells. Sequence comparisons and molecular modeling of 83D4 indicate that the antibody combining site displays a cavity-like feature primarily defined by the CDR H1 and H2 loops. This pocket could accommodate a single Tn molecule, thus, suggesting a structural explanation for the predominant expression of a particular VH gene segment in a group of antibodies that recognize tumor-associated antigens arising from an aberrant O-glycosylation.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03136640
Contributor : Marie de Tarragon <>
Submitted on : Tuesday, February 9, 2021 - 7:09:52 PM
Last modification on : Friday, April 2, 2021 - 11:36:00 AM

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Alvaro Babino, Otto Pritsch, Pablo Oppezzo, Renaud Du Pasquier, Alberto Roseto, et al.. Molecular Cloning of a Monoclonal Anti-Tumor Antibody Specific for the Tn Antigen and Expression of an Active Single-Chain Fv Fragment. HYBRIDOMA, Mary Ann Liebert, 1997, 16 (4), pp.317-324. ⟨10.1089/hyb.1997.16.317⟩. ⟨pasteur-03136640⟩

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