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The human pre-B cell receptor: Structural constraints for a tentative model of the pseudo-light (ΨL) chain

Abstract : In human pre-B cells, the mu chain is associated with a surrogate light chain composed of the lambda-like and Vpre-B gene products. This pre-B cell receptor presumably triggers early steps of B cell differentiation, We have determined the NH2-terminal amino acid sequence of the lambda-like chain, showing that the mature chain results from the cleavage of a leader segment of 44 residues, leaving a polypeptide of 169 amino acids having partial features of the Ig light chain domains, with the exception of the first 50 amino acid NH2-terminal region. We have completed the nucleotide sequence of the Vpre-B gene, which appears to contain 126 residues in its mature form of which the 24 COOH-terminal portion was not Ig-related. Analysis of transfectants has provided direct evidence that lambda-like and Vpre-B chains assemble together even in the absence of heavy chain, prompting the search for a structural basis of this interaction. Comparison with the domain organization of the regular Ig lambda chain suggests that most of the psi L chain can be accommodated within a CL-VL-like structure, with an extra "subdomain" contributed by the non-Ig-like portions of both the lambda-like and Vpre-B polypeptides.
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Contributor : Marie de Tarragon <>
Submitted on : Tuesday, February 9, 2021 - 5:59:30 PM
Last modification on : Monday, April 12, 2021 - 3:52:04 PM

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V. Guelpa-Fonlupt, D. Bossy, P. Alzari, F. Fumoux, M. Fougereau, et al.. The human pre-B cell receptor: Structural constraints for a tentative model of the pseudo-light (ΨL) chain. Molecular Immunology, Elsevier, 1994, 31 (14), pp.1099-1108. ⟨10.1016/0161-5890(94)90105-8⟩. ⟨pasteur-03136560⟩

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