Three-dimensional structure of two crystal forms of FabR19.9 from a monoclonal anti-arsonate antibody. - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 1992

Three-dimensional structure of two crystal forms of FabR19.9 from a monoclonal anti-arsonate antibody.

Résumé

The three-dimensional structure of FabR19.9 from a well-characterized anti-p-azobenzenearsonate monoclonal antibody has been determined by x-ray diffraction techniques in two crystalline forms (I and II) to a resolution of 2.8 and 2.7 A, respectively. Essentially the same tertiary and quaternary structure of the Fab is observed in the two forms. The major difference resides in the intermolecular contacts, which are interpreted to favor an irreversible transition from the metastable form I to the more stable form II. The third complementarity-determining region of the heavy chain (H3) folds back over the combining site and requires rearrangement for hapten binding. This dynamic requirement on H3 is consistent with its mobility in the structure and can explain hapten binding to an otherwise inaccessible antibody combining site.

Dates et versions

pasteur-03136506 , version 1 (09-02-2021)

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Marie-Bernard Lascombe, Pedro M. Alzari, Roberto J. Poljak, Alfred Nisonoff. Three-dimensional structure of two crystal forms of FabR19.9 from a monoclonal anti-arsonate antibody.. Proceedings of the National Academy of Sciences of the United States of America, 1992, 89 (20), pp.9429-33. ⟨10.1073/pnas.89.20.9429⟩. ⟨pasteur-03136506⟩

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