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Diagnostic Assays for Crimean-Congo Hemorrhagic Fever

Abstract : Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, in-house methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specificity was excellent for all assays; molecular test results were influenced by patient country of origin. Our findings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03112961
Contributor : Jessica Vanhomwegen <>
Submitted on : Monday, January 18, 2021 - 1:49:25 AM
Last modification on : Thursday, February 4, 2021 - 3:06:45 AM
Long-term archiving on: : Monday, April 19, 2021 - 6:25:56 PM

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Distributed under a Creative Commons Attribution 4.0 International License

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Jessica Vanhomwegen, Maria João Alves, Tatjana Avšič Županc, Silvia Bino, Sadegh Chinikar, et al.. Diagnostic Assays for Crimean-Congo Hemorrhagic Fever. Emerging Infectious Diseases, Centers for Disease Control and Prevention, 2012, 18 (12), pp.1958-1965. ⟨10.3201/eid1812.120710⟩. ⟨pasteur-03112961⟩

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