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mSphere of Influence: Expanding the CRISPR Sphere with Single-Locus Proteomics

Abstract : Lucy Glover's research focuses on the role of DNA repair and recombination in antigenic variation in the parasite Trypanosoma brucei, the causative agent of both human and animal African trypanosomiasis. In this mSphere of Influence article, she reflects on how "A CRISPR-based approach for proteomic analysis of a single genomic locus" by Z. J. Waldrip, S. D. Byrum, A. J. Storey, J. Gao, et al. (Epigenetics 9:1207-1211, 2014, https://doi.org/10.4161/epi.29919) made an impact on her research by taking the precision of CRISPR-Cas9 and repurposing it to look at single-locus proteomics. By using this technology in trypanosomes, Dr. Glover and her colleagues could study the dynamic accumulation of repair proteins after specific damage and gain insight into how the location of a double-strand break (DSB) dictates repair pathway choice and how this may influence immune evasion in these parasites.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-03107138
Contributor : Lucy Glover <>
Submitted on : Tuesday, January 12, 2021 - 1:24:51 PM
Last modification on : Thursday, January 21, 2021 - 3:29:33 AM
Long-term archiving on: : Tuesday, April 13, 2021 - 6:33:20 PM

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Distributed under a Creative Commons Attribution 4.0 International License

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Lucy Glover. mSphere of Influence: Expanding the CRISPR Sphere with Single-Locus Proteomics. MSphere, American Society for Microbiology., 2020, 5 (1), ⟨10.1128/mSphere.00001-20⟩. ⟨pasteur-03107138⟩

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