Mass‐spectrometry analysis of the human pineal proteome during night and day and in autism

Guillaume Dumas (1, 2) , Hany Goubran‐botros (1) , Mariette I Matondo (3) , Cécile Pagan (4, 5) , Cyril Boulègue (3) , Thibault Chaze (3) , Julia Chamot-Rooke (3) , Erik Maronde (6) , Thomas Bourgeron (1)

1 GHFC (UMR_3571 / U-Pasteur_1) - Génétique humaine et fonctions cognitives - Human Genetics and Cognitive Functions
2 PPHP - Precision Psychiatry and Social Physiology laboratory [Montreal]
3 UTechS MSBio - Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology
4 USPC - Université Sorbonne Paris Cité
5 Inserm U942 - Service de Biochimie et de Biologie Moléculaire [AP-HP Hôpital Lariboisière]
6 Institute for Anatomy II

The human pineal gland regulates day‐night dynamics of multiple physiological processes, especially through the secretion of melatonin. Using mass spectrometry‐based proteomics and dedicated analysis tools, we identify proteins in the human pineal gland and analyze systematically their variation throughout the day, and, compare these changes in the pineal proteome between control specimens and donors diagnosed with autism. Results reveal diverse regulated clusters of proteins with, among others, catabolic carbohydrate process and cytoplasmic membrane‐bounded vesicle‐related proteins differing between day and night and/or control versus autism pineal glands. These data show novel and unexpected processes happening in the human pineal gland during the day/night rhythm as well as specific differences between autism donor pineal glands and those from controls.

Keywords: antioxidant; autism spectrum disorder; chaperon proteins; circadian rhythm; energy production; mass spectometry; pineal gland

Domains: Life Sciences [q-bio]; Cellular Biology; Life Sciences [q-bio]; Biochemistry, Molecular Biology

Guillaume Dumas, Hany Goubran‐botros, Mariette I Matondo, Cécile Pagan, Cyril Boulègue, et al.. Mass‐spectrometry analysis of the human pineal proteome during night and day and in autism. Journal of Pineal Research, 2020, 70, ⟨10.1111/jpi.12713⟩. ⟨pasteur-03096880⟩