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Abstract : Recent advances in genomics have uncovered the tremendous diversity and richness of microbial ecosystems. New functional genomics methods are now needed to probe gene function in high-throughput and provide mechanistic insights. Here, we review how the CRISPR toolbox can be used to inactivate, repress or overexpress genes in a sequence-specific manner and how this offers diverse attractive solutions to identify gene function in high-throughput. Developed both in eukaryotes and prokaryotes, CRISPR screening technologies have already provided meaningful insights in microbiology and host-pathogen interactions. In the era of microbiomes, the versatility and the functional diversity of CRISPR-derived tools has the potential to significantly improve our understanding of microbial communities and their interaction with the host.
https://hal-pasteur.archives-ouvertes.fr/pasteur-02978339 Contributor : David BikardConnect in order to contact the contributor Submitted on : Monday, October 26, 2020 - 1:08:13 PM Last modification on : Thursday, April 7, 2022 - 10:10:43 AM Long-term archiving on: : Wednesday, January 27, 2021 - 6:48:58 PM
François Rousset, David Bikard. CRISPR screens in the era of microbiomes. Current Opinion in Microbiology, Elsevier, 2020, 57, pp.70-77. ⟨10.1016/j.mib.2020.07.009⟩. ⟨pasteur-02978339⟩