Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

CRISPR screens in the era of microbiomes

François Rousset 1, 2 David Bikard 1, * 
* Corresponding author
2 Collège Doctoral
SU - Sorbonne Université
Abstract : Recent advances in genomics have uncovered the tremendous diversity and richness of microbial ecosystems. New functional genomics methods are now needed to probe gene function in high-throughput and provide mechanistic insights. Here, we review how the CRISPR toolbox can be used to inactivate, repress or overexpress genes in a sequence-specific manner and how this offers diverse attractive solutions to identify gene function in high-throughput. Developed both in eukaryotes and prokaryotes, CRISPR screening technologies have already provided meaningful insights in microbiology and host-pathogen interactions. In the era of microbiomes, the versatility and the functional diversity of CRISPR-derived tools has the potential to significantly improve our understanding of microbial communities and their interaction with the host.
Document type :
Journal articles
Complete list of metadata

Cited literature [65 references]  Display  Hide  Download
Contributor : David Bikard Connect in order to contact the contributor
Submitted on : Monday, October 26, 2020 - 1:08:13 PM
Last modification on : Thursday, April 7, 2022 - 10:10:43 AM
Long-term archiving on: : Wednesday, January 27, 2021 - 6:48:58 PM


Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License




François Rousset, David Bikard. CRISPR screens in the era of microbiomes. Current Opinion in Microbiology, Elsevier, 2020, 57, pp.70-77. ⟨10.1016/j.mib.2020.07.009⟩. ⟨pasteur-02978339⟩



Record views


Files downloads