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Journal Articles Current Opinion in Microbiology Year : 2020

CRISPR screens in the era of microbiomes

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Abstract

Recent advances in genomics have uncovered the tremendous diversity and richness of microbial ecosystems. New functional genomics methods are now needed to probe gene function in high-throughput and provide mechanistic insights. Here, we review how the CRISPR toolbox can be used to inactivate, repress or overexpress genes in a sequence-specific manner and how this offers diverse attractive solutions to identify gene function in high-throughput. Developed both in eukaryotes and prokaryotes, CRISPR screening technologies have already provided meaningful insights in microbiology and host-pathogen interactions. In the era of microbiomes, the versatility and the functional diversity of CRISPR-derived tools has the potential to significantly improve our understanding of microbial communities and their interaction with the host.
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pasteur-02978339 , version 1 (26-10-2020)

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Attribution - CC BY 4.0

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François Rousset, David Bikard. CRISPR screens in the era of microbiomes. Current Opinion in Microbiology, 2020, 57, pp.70-77. ⟨10.1016/j.mib.2020.07.009⟩. ⟨pasteur-02978339⟩
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