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Journal articles
CRISPR screens in the era of microbiomes
Abstract : Recent advances in genomics have uncovered the tremendous diversity and richness of microbial ecosystems. New functional genomics methods are now needed to probe gene function in high-throughput and provide mechanistic insights. Here, we review how the CRISPR toolbox can be used to inactivate, repress or overexpress genes in a sequence-specific manner and how this offers diverse attractive solutions to identify gene function in high-throughput. Developed both in eukaryotes and prokaryotes, CRISPR screening technologies have already provided meaningful insights in microbiology and host-pathogen interactions. In the era of microbiomes, the versatility and the functional diversity of CRISPR-derived tools has the potential to significantly improve our understanding of microbial communities and their interaction with the host.
Cited literature [65 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-02978339
Contributor : David Bikard Connect in order to contact the contributor
Submitted on : Monday, October 26, 2020 - 1:08:13 PM
Last modification on : Monday, January 17, 2022 - 3:10:02 PM
Long-term archiving on: : Wednesday, January 27, 2021 - 6:48:58 PM
Contributor : David Bikard Connect in order to contact the contributor
Submitted on : Monday, October 26, 2020 - 1:08:13 PM
Last modification on : Monday, January 17, 2022 - 3:10:02 PM
Long-term archiving on: : Wednesday, January 27, 2021 - 6:48:58 PM
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Distributed under a Creative Commons Attribution 4.0 International License