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Isolation, Purification, and Characterization of Leptophages

Abstract : To date, only three bacteriophages of leptospires-leptophages-are known. Nonetheless, numerous prophages have been found in the genus, especially in the genomes of pathogenic species. Thus, some laboratories attempt to isolate leptophage particles from environmental samples or following mitomycin C induction of bacterial cultures. Here, we propose multiple procedures to isolate, purify, and characterize bacteriophages, based on protocols used for LE3 and LE4 characterization.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-02951927
Contributor : Sylvie Murguet <>
Submitted on : Tuesday, September 29, 2020 - 10:03:31 AM
Last modification on : Thursday, June 17, 2021 - 11:20:01 AM

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Olivier Schiettekatte, Pascale Bourhy. Isolation, Purification, and Characterization of Leptophages. Leptospira spp., 2134, Humana, pp.67-75, 2020, Methods in Molecular Biology, 978-1-0716-0459-5. ⟨10.1007/978-1-0716-0459-5_7⟩. ⟨pasteur-02951927⟩

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