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Isolation, Purification, and Characterization of Leptophages

Abstract : To date, only three bacteriophages of leptospires-leptophages-are known. Nonetheless, numerous prophages have been found in the genus, especially in the genomes of pathogenic species. Thus, some laboratories attempt to isolate leptophage particles from environmental samples or following mitomycin C induction of bacterial cultures. Here, we propose multiple procedures to isolate, purify, and characterize bacteriophages, based on protocols used for LE3 and LE4 characterization.
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Contributor : Sylvie Murguet Connect in order to contact the contributor
Submitted on : Tuesday, September 29, 2020 - 10:03:31 AM
Last modification on : Thursday, November 25, 2021 - 4:06:09 PM


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Olivier Schiettekatte, Pascale Bourhy. Isolation, Purification, and Characterization of Leptophages. Leptospira spp., 2134, Humana, pp.67-75, 2020, Methods in Molecular Biology, 978-1-0716-0459-5. ⟨10.1007/978-1-0716-0459-5_7⟩. ⟨pasteur-02951927⟩



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