Isolation, Purification, and Characterization of Leptophages

Olivier Schiettekatte (1, 2) , Pascale Bourhy (1)

1 Biologie des Spirochètes / Biology of Spirochetes
2 ED562 - BioSPC - École Doctorale Bio Sorbonne Paris Cité [Paris]

To date, only three bacteriophages of leptospires-leptophages-are known. Nonetheless, numerous prophages have been found in the genus, especially in the genomes of pathogenic species. Thus, some laboratories attempt to isolate leptophage particles from environmental samples or following mitomycin C induction of bacterial cultures. Here, we propose multiple procedures to isolate, purify, and characterize bacteriophages, based on protocols used for LE3 and LE4 characterization.

Mots clés: Leptophages; Polyethylene glycol precipitation; Cesium chloride purification; TEM characterization; One-step growth kinetics

Domaines: Microbiologie et Parasitologie

Olivier Schiettekatte, Pascale Bourhy. Isolation, Purification, and Characterization of Leptophages. Leptospira spp., 2134, Humana, pp.67-75, 2020, Methods in Molecular Biology, 978-1-0716-0459-5. ⟨10.1007/978-1-0716-0459-5_7⟩. ⟨pasteur-02951927⟩