A highly sensitive bioluminescent method for measuring allergen‐specific IgE in microliter samples

Sophie Goyard (1) , Bianca Balbino (2, 3) , Rebecca Chinthrajah (4, 5, 6) , Shu-Chen Lyu (4, 6) , Yves Louis Janin (7) , Pierre Bruhns (2) , Pascal Poncet (8, 9) , Stephen Galli (4, 6) , Kari Nadeau (4, 6) , Laurent L. Reber (2, 10) , Thierry Rose (1)

1 Biologie Cellulaire des Lymphocytes - Lymphocyte Cell Biology
2 Anticorps en thérapie et pathologie - Antibodies in Therapy and Pathology
3 SU - Sorbonne Université
4 Sean N. Parker Center for Allergy and Asthma Research [Stanford]
5 Stanford University
6 Stanford University School of Medicine [CA, USA]
7 Chimie et Biocatalyse
8 Département d'Immunologie - Department of Immunology
9 Service de Biochimie et de Biologie Moléculaire [CHU Trousseau]
10 CPTP - Centre de Physiopathologie Toulouse Purpan

Keywords: IgE; allergy diagnosis; bioluminescence; luciferase; nanobody; ImmunoCAP; ELISA

Domains: Immunology; Allergology

Sophie Goyard, Bianca Balbino, Rebecca Chinthrajah, Shu-Chen Lyu, Yves Louis Janin, et al.. A highly sensitive bioluminescent method for measuring allergen‐specific IgE in microliter samples. Allergy, 2020, 75 (11), pp.2952-2956. ⟨10.1111/all.14365⟩. ⟨pasteur-02949568⟩