A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions

Dae-Kyum Kim (1, 2) , Jennifer Knapp (1, 2) , Da Kuang (1, 2) , Aditya Chawla (1, 2) , Patricia Cassonnet (3) , Hunsang Lee (1) , Dayag Sheykhkarimli (1, 2) , Payman Samavarchi-Tehrani (2) , Hala Abdouni (1, 2) , Ashyad Rayhan (1, 2) , Roujia Li (1, 2) , Oxana Pogoutse (1, 2) , Étienne Coyaud (4) , Sylvie van Der Werf (3) , Caroline Demeret (3) , Anne-Claude Gingras (1, 2) , Mikko Taipale (1, 5) , Brian Raught (6) , Yves Jacob (3) , Frederick Roth (1, 2)

1 University of Toronto
2 Lunenfeld-Tanenbaum Research Institute [Toronto, Canada]
3 GMV-ARN (UMR_3569 / U-Pasteur_2) - Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses
4 PRISM - Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192
5 CIFAR - Canadian Institute for Advanced Research
6 Princess Margaret Cancer Centre [Toronto, Canada]

The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.

Keywords: Gateway-compatible; SARS-CoV-2; TEV (tobacco etch virus) sequence; coding sequence collection

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Microbiology and Parasitology; Virology; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Genomics [q-bio.GN]

Dae-Kyum Kim, Jennifer Knapp, Da Kuang, Aditya Chawla, Patricia Cassonnet, et al.. A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions. G3, 2020, 10 (9), pp.3399-3402. ⟨10.1534/g3.120.401554⟩. ⟨pasteur-02939727⟩