Yersinia artesiana sp. nov., Yersinia proxima sp. nov., Yersinia alsatica sp. nov., Yersinia vastinensis sp. nov., Yersinia thracica sp. nov. and Yersinia occitanica sp. nov., isolated from humans and animals

Anne-Sophie Le Guern (1, 2) , Cyril Savin (1, 2, 3) , Hilde Angermeier (2, 4) , Sylvie Brémont (1, 2) , Dominique Clermont (5) , Estelle Mühle (5) , Petya Orozova (6) , Hristo Najdenski (7) , Javier Pizarro-Cerdá (1, 2)

1 Yersinia
2 CNR - Centre National de Référence de la Peste et autres Yersinioses - National Reference Center Plague and Yersinioses
3 UPCité - Université Paris Cité
4 ECDC - European Centre for Disease Prevention and Control [Stockholm, Sweden]
5 CIP - Collection de l'Institut Pasteur
6 NDRVMI - National Diagnostic and Research Veterinary Medical Institute
7 Institut Stephan Angeloff

Thirty-three Yersinia strains previously characterized by the French Yersinia National Reference Laboratory (YNRL) and isolated from humans and animals were suspected to belong to six novel species by a recently described core genome multilocus sequence typing scheme. These strains and five additional strains from the YNRL were characterized using a polyphasic taxonomic approach including a phylogenetic analysis based on 500 core genes, determination of average nucleotide identity (ANI), determination of DNA G+C content and identification of phenotypic features. Phylogenetic analysis confirmed that the 38 studied strains formed six well-demarcated clades. ANI values between these clades and their closest relatives were <94.7 % and ANI values within each putative novel species were >97.5 %. Distinctive biochemical characteristics were identified in five out of the six novel species. All of these data demonstrated that the 38 strains belong to six novel species of the genus Yersinia: Yersinia artesiana sp. nov., type strain IP42281T (=CIP 111845T=DSM 110725T); Yersinia proxima sp. nov., type strain IP37424T (=CIP 111847T=DSM 110727T); Yersinia alsatica sp. nov., type strain IP38850T (=CIP 111848T=DSM 110726T); Yersinia vastinensis sp. nov., type strain IP38594T (=CIP 111844T=DSM 110738T); Yersinia thracica sp. nov., type strain IP34646T (=CIP 111842T=DSM 110736T); and Yersinia occitanica sp. nov., type strain IP35638T (=CIP 111843T=DSM 110739T).

Mots clés: Yersinia; Yersiniaceae; cgMLST; species

Domaines: Bactériologie; Microbiologie et Parasitologie

Anne-Sophie Le Guern, Cyril Savin, Hilde Angermeier, Sylvie Brémont, Dominique Clermont, et al.. Yersinia artesiana sp. nov., Yersinia proxima sp. nov., Yersinia alsatica sp. nov., Yersinia vastinensis sp. nov., Yersinia thracica sp. nov. and Yersinia occitanica sp. nov., isolated from humans and animals. International Journal of Systematic and Evolutionary Microbiology, 2020, ⟨10.1099/ijsem.0.004417⟩. ⟨pasteur-02936765⟩