A Dual Protein-mRNA Localization Screen Reveals Compartmentalized Translation and Widespread Co-translational RNA Targeting - Archive ouverte HAL Access content directly
Journal Articles Developmental Cell Year : 2020

A Dual Protein-mRNA Localization Screen Reveals Compartmentalized Translation and Widespread Co-translational RNA Targeting

(1, 2) , (1, 2) , (1) , (3, 4) , (5) , (6, 7) , (1) , (1) , (1) , (1) , (8) , (6, 7) , (8) , (5) , (2) , (1) , (6, 7) , (3, 4) , (1, 9)
1
2
3
4
5
6
7
8
9
Oh Sung Kwon
Hervé Le Hir
Kazem Zibara

Abstract

Local translation allows spatial control of gene expression. Here, we performed a dual protein-mRNA localization screen, using smFISH on 523 human cell lines expressing GFP-tagged genes. 32 mRNAs displayed specific cytoplasmic localizations with local translation at unexpected locations, including cytoplasmic protrusions, cell edges, endosomes, Golgi, the nuclear envelope, and centrosomes, the latter being cell-cycle-dependent. Automated classification of mRNA localization patterns revealed a high degree of intercellular heterogeneity. Surprisingly, mRNA localization frequently required ongoing translation, indicating widespread co-translational RNA targeting. Interestingly, while P-body accumulation was frequent (15 mRNAs), four mRNAs accumulated in foci that were distinct structures. These foci lacked the mature protein, but nascent polypeptide imaging showed that they were specialized translation factories. For β-catenin, foci formation was regulated by Wnt, relied on APC-dependent polysome aggregation, and led to nascent protein degradation. Thus, translation factories uniquely regulate nascent protein metabolism and create a fine granular compartmentalization of translation.
Fichier principal
Vignette du fichier
Chouaib_et_al_HAL_2.pdf (15.65 Mo) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

pasteur-02925687 , version 1 (16-11-2020)

Licence

Attribution - NonCommercial - CC BY 4.0

Identifiers

Cite

Racha Chouaib, Adham Safieddine, Xavier Pichon, Arthur Imbert, Oh Sung Kwon, et al.. A Dual Protein-mRNA Localization Screen Reveals Compartmentalized Translation and Widespread Co-translational RNA Targeting. Developmental Cell, 2020, 54 (6), pp.773 - 791.e5. ⟨10.1016/j.devcel.2020.07.010⟩. ⟨pasteur-02925687⟩
255 View
416 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More