Skip to Main content Skip to Navigation
Journal articles

Role of a patatin-like phospholipase in Plasmodium falciparum gametogenesis and malaria transmission

Abstract : Transmission of Plasmodium falciparum involves a complex process that starts with the ingestion of gametocytes by female Anopheles mosquitoes during a blood meal. Activation of gametocytes in the mosquito midgut triggers "rounding up" followed by egress of both male and female gametes. Egress requires secretion of a perforin-like protein, PfPLP2, from intracellular vesicles to the periphery, which leads to destabilization of peripheral membranes. Male gametes also develop flagella, which assist in binding female gametes for fertilization. This process of gametogenesis, which is key to malaria transmission, involves extensive membrane remodeling as well as vesicular discharge. Phospholipase A2 enzymes (PLA2) are known to mediate membrane remodeling and vesicle secretion in diverse organisms. Here, we show that a P. falciparum patatin-like phospholipase (PfPATPL1) with PLA2 activity plays a key role in gametogenesis. Conditional deletion of the gene encoding PfPATPL1 does not affect P. falciparum blood stage growth or gametocyte development but reduces efficiency of rounding up, egress, and exflagellation of gametocytes following activation. Interestingly, deletion of the PfPATPL1 gene inhibits secretion of PfPLP2, reducing the efficiency of gamete egress. Deletion of PfPATPL1 also reduces the efficiency of oocyst formation in mosquitoes. These studies demonstrate that PfPATPL1 plays a role in gametogenesis, thereby identifying PLA2 phospholipases such as PfPATPL1 as potential targets for the development of drugs to block malaria transmission.
Document type :
Journal articles
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-02890967
Contributor : Audrey Lorthiois <>
Submitted on : Monday, July 6, 2020 - 4:37:10 PM
Last modification on : Tuesday, October 13, 2020 - 3:46:08 PM

Links full text

Identifiers

Citation

Pallavi Singh, Aditi Alaganan, Kunal More, Audrey Lorthiois, Sabine Thiberge, et al.. Role of a patatin-like phospholipase in Plasmodium falciparum gametogenesis and malaria transmission. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2019, 116 (35), pp.17498-17508. ⟨10.1073/pnas.1900266116⟩. ⟨pasteur-02890967⟩

Share

Metrics

Record views

45