Skip to Main content Skip to Navigation
Journal articles

Vibrio cholerae chromosome 2 copy number is controlled by the methylation-independent binding of its monomeric initiator to the chromosome 1 crtS site

Abstract : Bacteria contain a primary chromosome and, frequently , either essential secondary chromosomes or dispensable megaplasmids of plasmid origin. Incoming plasmids are often poorly adapted to their hosts and their stabilization requires integration with the host's cellular mechanisms in a process termed domestication. All Vibrio, including pathogenic species, carry a domesticated secondary chromosome (Chr2) where replication is coordinated with that of the primary chromosome (Chr1). Chr2 replication is triggered by the replication of an in-tergenic sequence (crtS) located on Chr1. Yet, the molecular mechanisms by which crtS replication controls the initiation of Chr2 replication are still largely unknown. In this study, we show that crtS not only regulates the timing of Chr2 initiation but also controls Chr2 copy number. We observed and characterized the direct binding of the Chr2 initia-tor (RctB) on crtS. RctB binding to crtS is independent of its methylation state. RctB molecules, which naturally form dimers, preferentially bind to crtS as monomers, with DnaK/J protein chaperones shown to stimulate binding of additional RctB monomers on crtS. In this study, we addressed various hypothesis of how replication of crtS could trigger Chr2 replica-tion and provide new insights into its mode of action.
Complete list of metadatas

Cited literature [42 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-02890948
Contributor : Isabelle Hoffmann <>
Submitted on : Monday, July 6, 2020 - 4:25:02 PM
Last modification on : Thursday, July 9, 2020 - 3:37:00 AM

File

2018 Nucleic Acids Res_de Lemo...
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License

Identifiers

Collections

Citation

Francisco de Lemos Martins, Florian Fournes, Maria-Vittoria Mazzuoli, Didier Mazel, Marie-Eve Val. Vibrio cholerae chromosome 2 copy number is controlled by the methylation-independent binding of its monomeric initiator to the chromosome 1 crtS site. Nucleic Acids Research, Oxford University Press, 2018, 46 (19), pp.10145-10156. ⟨10.1093/nar/gky790⟩. ⟨pasteur-02890948⟩

Share

Metrics

Record views

21

Files downloads

49