icsB: a Shigella flexneri virulence gene necessary for the lysis of protrusions during intercellular spread - Archive ouverte HAL Access content directly
Journal Articles Molecular Microbiology Year : 1992

icsB: a Shigella flexneri virulence gene necessary for the lysis of protrusions during intercellular spread

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Abstract

Shigella flexneri causes baclllary dysentery by invading epithelial cells of the colonic mucosa. We have characterized the icsB gene which is located on the virulence plasmid pWRIOO. After inactivation of icsB, the mutant strain remained invasive, but formed abnormally small plaques on HeLa cell monolayers, colonized only the peripheral cells of Caco-2 islets, and was unable to provoke a keratoconjunctivitis in guinea-pigs. Examination of infected HeLa cells showed that the icsB mutant was able to lyse the phagocytic vacuole and to form protrusions at the surface of infected cells, but, unlike the wild type, remained trapped in protrusions surrounded by two membranes. These results indicate that IcsB is involved in the lysis of the protrusions, a step necessary for intercellular spread.
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pasteur-02884236 , version 1 (29-06-2020)

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Abdelmounaaïm Allaoui, Joelle Mounier, Marie-Christine Prévost, Philippe Sansonetti, Claude Parsot. icsB: a Shigella flexneri virulence gene necessary for the lysis of protrusions during intercellular spread. Molecular Microbiology, 1992, 6 (12), pp.1605-1616. ⟨10.1111/j.1365-2958.1992.tb00885.x⟩. ⟨pasteur-02884236⟩

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