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Journal articles
Accelerated thymopoiesis and improved T‐cell responses in HLA‐A2/‐DR2 transgenic BRGS‐based human immune system mice
Guillemette Masse-Ranson
1, 2
Mathilde Dusseaux
1
Oriane Fiquet
1
Sylvie Darche
1
Maud Boussand
1
Yan Li
1
Silvia Lopez-Lastra
1
Nicolas Legrand
Erwan Corcuff
Antoine Toubert
3, 4, 5
Mireille Centlivre
2
Timothée Bruel
6, 2
Hergen Spits
7
Olivier Schwartz
6, 2
Yves Levy
2, 8, 9
Helene Strick-Marchand
1
James Di Santo
1, *
*
Corresponding author
9
Service d'immunologie clinique et maladies infectieuses [Créteil]
Groupe Henri Mondor-Albert Chenevier
Guillemette Masse-Ranson 1, 2
Author
Mathilde Dusseaux 1
Author
Oriane Fiquet 1
Author
Sylvie Darche 1
Author
Erwan Corcuff
Author
Antoine Toubert 3, 4, 5
Author
Mireille Centlivre 2
Author
Timothée Bruel 6, 2
Author IdHAL : timothee-bruel ORCID : https://orcid.org/0000-0002-3952-4261
Hergen Spits 7
Author
Olivier Schwartz 6, 2
Author
Yves Levy 2, 8, 9
Author
Helene Strick-Marchand 1
Author
James P Di Santo 1
Correspondent author
1
Immunité Innée - Innate Immunity (Département d'Immunologie, 25-28 rue du Docteur Roux, 75724 Paris Cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1223 (101, rue de Tolbiac, 75013 Paris - France)
2
VRI - Vaccine Research Institute (61 avenue du Général de Gaulle 94010 Créteil Cedex France - France)
- UPEC UP12 - Université Paris-Est Créteil Val-de-Marne - Paris 12 (61 avenue du Général de Gaulle - 94010 Créteil cedex - France)
3
Service d'Immunologie et d'Histocompatibilité (1, avenue Claude-Vellefaux 75010 Paris - France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris] (200 rue du Faubourg Saint-Denis 75475 Paris (Nord) Cedex 10 - France)
- UP - Université de Paris (85 boulevard Saint-Germain 75006 Paris - France)
4
IUH - Institut Universitaire d'Hématologie (Université Paris Diderot, 1 Av. Claude Vellefaux 75010 Paris - France)
- UPD7 - Université Paris Diderot - Paris 7 (5 rue Thomas-Mann - 75205 Paris cedex 13 - France)
5
EMily (UMR_S_1160 / U1160) - Ecotaxie, microenvironnement et développement lymphocytaire (Hôpital Saint-Louis, 1 avenue Claude Vellfaux, IUH, Centre Georges Hayem, 75010 PARIS - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1160 (101, rue de Tolbiac, 75013 Paris - France)
- UPD7 - Université Paris Diderot - Paris 7 : UMR_S_1160 (5 rue Thomas-Mann - 75205 Paris cedex 13 - France)
6
Virus et Immunité (28, rue du docteur Roux F-75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : URA3015 (France)
7
AMC - Academic Medical Center - Academisch Medisch Centrum [Amsterdam] (Meibergdreef 9 1105 AZ Amsterdam - Netherlands)
- UvA - University of Amsterdam [Amsterdam] (Spui 21 1012 WX Amsterdam - Netherlands)
8
IMRB - Institut Mondor de Recherche Biomédicale (8 rue du Général Sarrail 94010 Créteil - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U955 (101, rue de Tolbiac, 75013 Paris - France)
- IFR10 (France)
- UPEC UP12 - Université Paris-Est Créteil Val-de-Marne - Paris 12 : UMR955 (61 avenue du Général de Gaulle - 94010 Créteil cedex - France)
9
Service d'immunologie clinique et maladies infectieuses [Créteil] (51 avenue du Maréchal de Lattre de Tassigny
94000 Créteil - France)
- Groupe Henri Mondor-Albert Chenevier (France)
- AP-HP - Assistance publique - Hôpitaux de Paris (AP-HP) (France)
- Hôpital Henri Mondor (France)
- Hôpital Albert Chenevier (France)
Abstract : Human immune system (HIS) mouse models provide a robust in vivo platform to study human immunity. Nevertheless, the signals that guide human lymphocyte differentiation in HIS mice remain poorly understood. Here, we have developed a novel Balb/c Rag2-/- Il2rg-/- SirpaNOD (BRGS) HIS mouse model expressing human HLA-A2 and -DR2 transgenes (BRGSA2DR2). When comparing BRGS and BRGSA2DR2 HIS mice engrafted with human CD34+ stem cells, a more rapid emergence of T cells in the circulation of hosts bearing human HLA was shown, which may reflect a more efficient human T-cell development in the mouse thymus. Development of CD4+ and CD8+ T cells was accelerated in BRGSA2DR2 HIS mice and generated more balanced B and T-cell compartments in peripheral lymphoid organs. Both B- and T-cell function appeared enhanced in the presence of human HLA transgenes with higher levels of class switched Ig, increased percentages of polyfunctional T cells and clear evidence for antigen-specific T-cell responses following immunization. Taken together, the presence of human HLA class I and II molecules can improve multiple aspects of human B- and T-cell homeostasis and function in the BRGS-based HIS mouse model.
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Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-02875581
Contributor : Helene Strick-Marchand <>
Submitted on : Friday, June 19, 2020 - 6:14:15 PM
Last modification on : Tuesday, June 8, 2021 - 11:06:03 AM
Contributor : Helene Strick-Marchand <>
Submitted on : Friday, June 19, 2020 - 6:14:15 PM
Last modification on : Tuesday, June 8, 2021 - 11:06:03 AM
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Identifiers
- HAL Id : pasteur-02875581, version 1
- DOI : 10.1002/eji.201848001
- PUBMED : 30888052
Collections
PASTEUR | UPEC-UPEM | UPEC | ATP-IMMUN | CNRS | UNIV-PARIS7 | UP-SANTE | IMRB | USPC | UNIV-PARIS
Citation
Guillemette Masse-Ranson, Mathilde Dusseaux, Oriane Fiquet, Sylvie Darche, Maud Boussand, et al.. Accelerated thymopoiesis and improved T‐cell responses in HLA‐A2/‐DR2 transgenic BRGS‐based human immune system mice. European Journal of Immunology, Wiley-VCH Verlag, 2019, 49 (6), pp.954-965. ⟨10.1002/eji.201848001⟩. ⟨pasteur-02875581⟩
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