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B lymphocytes undergo TLR2-dependent apoptosis upon Shigella infection

Abstract : Antibody-mediated immunity to Shigella, the causative agent of bacillary dysentery, requires several episodes of infection to get primed and is short-lasting, suggesting that the B cell response is functionally impaired. We show that upon ex vivo infection of human colonic tissue, invasive S. flexneri interacts with and occasionally invades B lymphocytes. The induction of a type three secretion apparatus (T3SA)-dependent B cell death is observed in the human CL-01 B cell line in vitro, as well as in mouse B lymphocytes in vivo. In addition to cell death occurring in Shigella-invaded CL-01 B lymphocytes, we provide evidence that the T3SA needle tip protein IpaD can induce cell death in noninvaded cells. IpaD binds to and induces B cell apoptosis via TLR2, a signaling receptor thus far considered to result in activation of B lymphocytes. The presence of bacterial co-signals is required to sensitize B cells to apoptosis and to up-regulate tlr2, thus enhancing IpaD binding. Apoptotic B lymphocytes in contact with Shigella-IpaD are detected in rectal biopsies of infected individuals. This study therefore adds direct B lymphocyte targeting to the diversity of mechanisms used by Shigella to dampen the host immune response.
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Submitted on : Friday, June 19, 2020 - 12:11:45 PM
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B lymphocytes undergo TLR2-dep...
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Katharina Nothelfer, Ellen T Arena, Laurie Pinaud, Michel Neunlist, Brian Mozeleski, et al.. B lymphocytes undergo TLR2-dependent apoptosis upon Shigella infection. Journal of Experimental Medicine, 2014, 211 (6), pp.1215-1229. ⟨10.1084/jem.20130914⟩. ⟨pasteur-02874944⟩



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