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, 6 dpf (corresponding to 0 dpi) GF or Conv zebrafish larvae were exposed to different 1063 doses of F. columnare ALG by bath immersion and transferred after 3h into sterile water

, Mean survival is represented by a thick horizontal bar with standard deviation. For each 1065 condition, n = 12 zebrafish larvae. Larvae mortality rate was monitored daily and 1066 surviving fish were euthanized at day 9 post infection. Statistics correspond to unpaired, 1067 non-parametric Mann-Whitney test comparing all conditions to non

;. Conv,

, Blue mean bars correspond to non-exposed larvae and red mean bars correspond to larvae 1070 exposed to F. columnare. 1071 1072 1073 1074 1075 (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint this version posted, 2020.


, Figure 5. Intestine of infected germ-free zebrafish displays severe disorganization

. Germ-free, A: Representative picture 1124 of intestines of non-infected larvae. Fish were fixed for histology analysis or electron 1125 microscopy at 7 dpf. B: Representative picture of intestines of infected larvae exposed 1126 at 7 dpf to F.columnare ALG . In A and B: Left column: Toluidine blue staining of Epon-1127 embedded zebrafish larvae for Light microscopy. Right column: Transmission electron 1128 microscopy. at 7 dpf (right). L= intestinal lumen. 1129 1130 1131 (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission, conventional and re-conventionalized zebrafish larvae. Re-conventionalized 1123 zebrafish were inoculated at 4 dpf with Mix9 or C. massiliae, 2020.