Fluorescent nanodiamond tracking reveals intraneuronal transport abnormalities induced by brain-disease-related genetic risk factors - Archive ouverte HAL Access content directly
Journal Articles Nature Nanotechnology Year : 2017

Fluorescent nanodiamond tracking reveals intraneuronal transport abnormalities induced by brain-disease-related genetic risk factors

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Nitin Mohan
  • Function : Author
  • PersonId : 1007661
Yann Loe-Mie
Marie-Pierre Adam
  • Function : Author
  • PersonId : 1004518
Christiane Rose
  • Function : Author
  • PersonId : 928909

Abstract

Brain diseases such as autism and Alzheimer's disease (each inflicting >1% of the world population) involve a large network of genes displaying subtle changes in their expression. Abnormalities in intraneuronal transport have been linked to genetic risk factors found in patients, suggesting the relevance of measuring this key biological process. However, current techniques are not sensitive enough to detect minor abnormalities. Here we report a sensitive method to measure the changes in intraneuronal transport induced by brain-disease-related genetic risk factors using fluorescent nanodiamonds (FNDs). We show that the high brightness, photostability and absence of cytotoxicity allow FNDs to be tracked inside the branches of dissociated neurons with a spatial resolution of 12 nm and a temporal resolution of 50 ms. As proof of principle, we applied the FND tracking assay on two transgenic mouse lines that mimic the slight changes in protein concentration (∼30%) found in the brains of patients. In both cases, we show that the FND assay is sufficiently sensitive to detect these changes.
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Dates and versions

pasteur-02873510 , version 1 (22-12-2020)

Licence

Attribution - NonCommercial - CC BY 4.0

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Cite

Simon Haziza, Nitin Mohan, Yann Loe-Mie, Aude-Marie Lepagnol-Bestel, Sophie Massou, et al.. Fluorescent nanodiamond tracking reveals intraneuronal transport abnormalities induced by brain-disease-related genetic risk factors. Nature Nanotechnology, 2017, 12 (4), pp.322-328. ⟨10.1038/nnano.2016.260⟩. ⟨pasteur-02873510⟩
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