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Journal articles
Fluorescent nanodiamond tracking reveals intraneuronal transport abnormalities induced by brain-disease-related genetic risk factors
Simon Haziza
1, 2, *
Nitin Mohan
1
Yann Loe-Mie
2
Aude-Marie Lepagnol-Bestel
2
Sophie Massou
1
Marie-Pierre Adam
1
Xuan Loc Le
1
Julia Viard
2
Christine Plancon
3
Rachel Daudin
2
Pascale Koebel
4
Emilie Dorard
2
Christiane Rose
2
Feng-Jen Hsieh
5
Chih-Che Wu
6
Brigitte Potier
2
Yann Hérault
4
Carlo Sala
7
Aiden Corvin
8
Bernadette Allinquant
2
Huan-Cheng Chang
5
François Treussart
1, *
Michel Simonneau
1, 2, *
*
Corresponding author
Simon Haziza 1, 2
Correspondent author
Nitin Mohan 1
Author
Yann Loe-Mie 2
Author IdHAL : yloemie ORCID : https://orcid.org/0000-0003-1427-8014
Aude-Marie Lepagnol-Bestel 2
Author
Christiane Rose 2
Author
Feng-Jen Hsieh 5
Author
Chih-Che Wu 6
Author
Brigitte Potier 2
Author IdHAL : brigitte-potier IdRef : https://www.idref.fr/160555248 ORCID : https://orcid.org/0000-0002-9235-7298
Yann Hérault 4
Author IdHAL : yann-herault ORCID : https://orcid.org/0000-0001-7049-6900
Carlo Sala 7
Author
Aiden Corvin 8
Author
Bernadette Allinquant 2
Author
Huan-Cheng Chang 5
Author
François Treussart 1
Correspondent author IdHAL : francois-treussart ORCID : https://orcid.org/0000-0002-1755-0688
Michel Simonneau 1, 2
Correspondent author
1
LAC - Laboratoire Aimé Cotton (France)
- CNRS - Centre National de la Recherche Scientifique : UPR3321 (France)
- UP11 - Université Paris-Sud - Paris 11 (Bâtiment 300 - 91405 Orsay cedex - France)
- ENS Cachan - École normale supérieure - Cachan (61, avenue du Président Wilson - 94230 Cachan - France)
2
U894 / UMS 1266 - Institut de psychiatrie et neurosciences (Centre de Psychiatrie et Neurosciences - Inserm U894
102-108 Rue de la Santé
75014 PARIS - France)
- UPD5 - Université Paris Descartes - Paris 5 : UMS 1266 (12, rue de l'École de Médecine - 75270 Paris cedex 06 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : UMS_S 894 (101, rue de Tolbiac, 75013 Paris - France)
3
IG - Institut de Génomique d'Evry (2 rue Gaston Crémieux CP5706 91057 EVRY CEDEX - France)
- JACOB - Institut de Biologie François JACOB : DRF/JACOB (DRF
CEA
Fontenay-aux-Roses - France)
- DRF (CEA) - Direction de Recherche Fondamentale (CEA) : DRF/JACOB (Direction de Recherche Fondamentale
Fundamental Research Division
CEA Saclay
91191 Gif-sur-Yvette
- France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- DRF (CEA) - Direction de Recherche Fondamentale (CEA) : DRF/JACOB (Direction de Recherche Fondamentale
Fundamental Research Division
CEA Saclay
91191 Gif-sur-Yvette
- France)
- Université Paris-Saclay (Bâtiment Bréguet, 3 Rue Joliot Curie 2e ét, 91190 Gif-sur-Yvette - France)
4
IGBMC - Institut de Génétique et de Biologie Moléculaire et Cellulaire (Parc D'Innovation 1 Rue Laurent Fries - BP 10142 67404 Illkirch Cedex - France)
- UNISTRA - Université de Strasbourg : UMR7104 (4 rue Blaise Pascal - CS 90032 - 67081 Strasbourg cedex - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1258 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : UMR7104 (France)
5
IAMS - Institute of Atomic and Molecular Sciences [Taipei] ( Academia Sinica, Taipei 10617, Taiwan - Taiwan)
- Academia Sinica (128 Academia Road, Section 2, Nankang, Taipei 11529 - Taiwan)
6
NCNU - National Chi Nan University (University Rd.Puli,Nantou Hsien, Taiwan 545 Republic of China - Taiwan)
- Chi Nan University (France)
7
Istituto di Neuroscienze (Istituto Di Neuroscienze - CNR, Via Giuseppe Moruzzi,1 - 56124 - Pisa - Italy)
8
Trinity College Dublin (College Green, Dublin 2, Ireland - Ireland)
Abstract : Brain diseases such as autism and Alzheimer's disease (each inflicting >1% of the world population) involve a large network of genes displaying subtle changes in their expression. Abnormalities in intraneuronal transport have been linked to genetic risk factors found in patients, suggesting the relevance of measuring this key biological process. However, current techniques are not sensitive enough to detect minor abnormalities. Here we report a sensitive method to measure the changes in intraneuronal transport induced by brain-disease-related genetic risk factors using fluorescent nanodiamonds (FNDs). We show that the high brightness, photostability and absence of cytotoxicity allow FNDs to be tracked inside the branches of dissociated neurons with a spatial resolution of 12 nm and a temporal resolution of 50 ms. As proof of principle, we applied the FND tracking assay on two transgenic mouse lines that mimic the slight changes in protein concentration (∼30%) found in the brains of patients. In both cases, we show that the FND assay is sufficiently sensitive to detect these changes.
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Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-02873510
Contributor : Yann Loe-Mie Connect in order to contact the contributor
Submitted on : Tuesday, December 22, 2020 - 12:08:34 AM
Last modification on : Friday, January 7, 2022 - 3:52:45 AM
Contributor : Yann Loe-Mie Connect in order to contact the contributor
Submitted on : Tuesday, December 22, 2020 - 12:08:34 AM
Last modification on : Friday, January 7, 2022 - 3:52:45 AM
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Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License
Identifiers
- HAL Id : pasteur-02873510, version 1
- DOI : 10.1038/nnano.2016.260
- PUBMED : 27893730
Collections
PASTEUR | ANR | JACOB | CEA-DRF | SITE-ALSACE | UNIV-PARIS | UP-SANTE | USPC | CEA-UPSAY | CEA | UNIV-PARIS5 | CNRS | ENS-CACHAN | IGBMC | LUMIN | INSERM | ENS-PARIS-SACLAY | GS-ENGINEERING
Citation
Simon Haziza, Nitin Mohan, Yann Loe-Mie, Aude-Marie Lepagnol-Bestel, Sophie Massou, et al.. Fluorescent nanodiamond tracking reveals intraneuronal transport abnormalities induced by brain-disease-related genetic risk factors. Nature Nanotechnology, Nature Publishing Group, 2017, 12 (4), pp.322-328. ⟨10.1038/nnano.2016.260⟩. ⟨pasteur-02873510⟩
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