Skip to Main content Skip to Navigation
Preprints, Working Papers, ...

A localization screen reveals translation factories and widespread co-translational RNA targeting

Abstract : Local translation allows a spatial control of gene expression. Here, we performed a dual protein/mRNA localization screen, using smFISH on 523 human cell lines expressing GFP-tagged genes. A total of 32 mRNAs displayed specific cytoplasmic localizations, and we observed local translation at unexpected locations, including cytoplasmic protrusions, cell edges, endosomes, Golgi, the nuclear envelope and centrosomes, the latter being cell cycle dependent. Quantitation of mRNA distribution and automatic pattern classification revealed a high degree of localization heterogeneity between cells. Surprisingly, mRNA localization frequently required ongoing translation, indicating widespread co-translational RNA targeting. Interestingly, while P-body accumulation was frequent (15 mRNAs), four mRNAs accumulated in foci that were distinct structures. These foci lacked the mature protein, but nascent polypeptide imaging showed that they were specialized translation factories. For β-catenin, foci formation was regulated by Wnt, relied on APC-dependent polysome aggregation, and led to nascent protein degradation. Thus, translation factories uniquely regulate nascent protein metabolism and create a fine granular compartmentalization of translation.
Document type :
Preprints, Working Papers, ...
Complete list of metadatas

Cited literature [18 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-02873144
Contributor : Marie Lemesle <>
Submitted on : Thursday, June 18, 2020 - 10:25:33 AM
Last modification on : Thursday, September 24, 2020 - 5:06:02 PM

File

2020.05.20.106989v1.full (1).p...
Files produced by the author(s)

Licence


Copyright

Identifiers

Citation

Racha Chouaib, Adham Safieddine, Xavier Pichon, Arthur Imbert, Oh Sung Kwon, et al.. A localization screen reveals translation factories and widespread co-translational RNA targeting. 2020. ⟨pasteur-02873144⟩

Share

Metrics

Record views

84

Files downloads

151