A complete list of all DNA-plasmids used in this study is provided in Supplementary Data 2. N-terminally FLAG-tagged MTMR13 in a pcDNA3.1 backbone was a kind gift from Gilbert di Paoli and was transferred into a pcDNA3.1-HA backbone, removing the HA-tag, using 5?-KpnI and 3?-EcoRV restriction sites. For the construction of mCherry-MTMR13, FLAG-tag was exchanged by mCherry from pmCherryC1 using restriction sites 5?-KpnI and 3?-EcoRV For mCherry-tagged MTMR13 domains, full-length MTMR13 (FL; aa1-1848; 5547 bp) was used as a PCR template. The DENN-domain truncation mutant of MTMR13 (MTMR13?DENN) and the various MTMR13 domains were amplified using primers specified in the primer table, p.1479 ,
, with restriction sites 5?-EcoRV and 3?-HindIII to obtain pcDNA3.1_BirA*-Rab35. pcDNA3.1_eGFP-Rab35 was generated by insertion of amplified Rab35 C-terminal to eGFP using restriction sites 5?-XhoI and 3?-EcoRI. pcDNA3.1_eGFP-Rab35CA(Q67L) and_eGFP-Rab35DN(S22N) were generated by QuickChange site-directed mutagenesis (Agilent). pEGFPC2_mCherry-MTMR5 was obtained by exchanging the Nterminal eGFP-tag for mCherry in pEGFPC2_GFP-MTMR5 (gift from Michael Clague) at restriction sites 5?-AgeI and 3?-HindIII. GST-Rab1A, -Rab5, -Rab7, and -Rab11 were a gift from Dr, PTP + coiled coil (CC) region (aa1100-1591): 1797 bp; PH domain (aa1743-1849): 324 bp. The respective PCR products were inserted into a pcDNA3.1-based mCherry-containing vector using restriction sites 5?-NotI and 3?-XbaI for MTMR13?DENN, 5?-EcoRV and 3?-NotI for DENN and PH-GRAM domains. For PTP, PTP + CC region and PH domains, pp.6-8
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