, Sodium chloride (NaCl), p.31434

, Magnesium chloride hexahydrate (MgCl2·6H2O), p.13152

, Polyethylene glycol (PEG), vol.600, p.87333

. Mes-(sigma-aldrich, catalog number: M8250)

, Magnesium sulfate (MgSO4)

(. Glycerol and . Applichem, , p.131339

, Polyethylene glycol (PEG), vol.4000, p.95904

, Lithium sulfate (Li2SO4), p.203653

, Beryllium chloride (BeCl2) (Sigma-Aldrich, catalog number: 201197) Note: This product has been discontinued

, Sodium fluoride (NaF), p.71519

, Polyethylene glycol (PEG), vol.3350, p.202444

, Tri-potassium citrate, p.1722

, Yeast extract (Sigma-Aldrich, catalog number: Y1625)

. Sigma-aldrich, Tryptone plus, p.61044

(. Agar and . Sigma-aldrich, , p.9799

, EDTA-free protease inhibitor cocktail tablets (Roche Diagnostics, p.11873580001

(. Imidazole and . Merck, , p.104716

, Dithiothreitol (Soltec Ventures, catalog number: M112)

, Hydrochloric acid solution, pp.13-1683

, Immobilized Metal Affinity Chromatography (IMAC) binding and washing buffer

, IMAC elution buffer (see Recipes)

, Dialysis buffer (see Recipes)

, Size Exclusion Chromatography buffer for the phosphatase complex (SEC-P buffer)

, Size Exclusion Chromatography buffer for the phosphotransferase complex (SEC-PT buffer) Equipment

, Pipetman P2 single channel pipette (Gilson, catalog number: F144801)

, Pipetman P20 single channel pipette (Gilson, catalog number: F123600)

, Pipetman P200 single channel pipette (Gilson, catalog number: F123601)

, Pipetman P1000 single channel pipette (Gilson, catalog number: F123602) 5. 250 ml Erlenmeyer flasks, p.4110207

C. Imelio,

, L Erlenmeyer flasks (Marienfeld-Superior, p.4110217

, HisTrap immobilized metal affinity chromatography (IMAC) 5 ml column with Zn-NTA resin (GE Healthcare, pp.17-5248

, Superdex S75 26/600 (GE Healthcare, p.28989334

, Stirring magnet

C. Thermomixer, . Eppendorf, ®. Thermomixer, and . Number, , pp.4053-8223

, Digital Ultrasonics Sonifier S-450 cell disruptor/homogenizer

, Multitron Standard incubation shaker (Infors HT)

, Minispin centrifuge (Eppendorf, model: MiniSpin ® , catalog number: 5452000018)

. Refrigerated-5424r-centrifuge, Eppendorf, model: 5424 R, catalog number, pp.36-102

. Refrigerated-5810r-centrifuge, Eppendorf, model: 5810 R, catalog number: 5811000010)

, Thermo Scientific TM , model: Sorvall Lynx4000, p.75006580

, Minipuls 3 peristaltic pump (Gilson, catalog number: F117604)

. Chromatography and . Healthcare, , p.18664501

, EPS-301 power supply (GE Healthcare, model: EPS 301, catalog number

, Cary 50 Bio UV-visible spectrophotometer

D. Alchemist and . Rigaku, Alchemist DT)

X. , generator MicroMax-007HF (Rigaku, model: MicroMax-007 HF)

, Multilayer X-ray mirrors Varimax-HF

, Image plate area detector MAR345 ® (marXperts, model: mar345)

, SZX16 microscope, p.16

. Evolt-e-, , vol.330, p.330

, LG-PS2 light source, p.2

, Software 1. Produced diffraction datasets: a. Complex DesKC:DesR-REC in phosphotransferase state, low Mg 2+

, Complex DesKC:DesR-REC in phosphotransferase state, high Mg, vol.2

, Complex DesKC:DesR-REC in phosphotransferase state, high Mg 2+ and BeF3

, Complex DesKC:DesR-REC in phosphatase state

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