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Journal articles
Histone H3 lysine 9 trimethylation and HP1γ favor inclusion of alternative exons
Abstract : Pre-messenger RNAs (pre-mRNAs) maturation is initiated cotranscriptionally. It is therefore conceivable that chromatin-borne information participates in alternative splicing. Here we find that elevated levels of trimethylation of histone H3 on Lys9 (H3K9me3) are a characteristic of the alternative exons of several genes including CD44. On this gene the chromodomain protein HP1γ, frequently defined as a transcriptional repressor, facilitates inclusion of the alternative exons via a mechanism involving decreased RNA polymerase II elongation rate. In addition, accumulation of HP1γ on the variant region of the CD44 gene stabilizes association of the pre-mRNA with the chromatin. Altogether, our data provide evidence for localized histone modifications impacting alternative splicing. They further implicate HP1γ as a possible bridging molecule between the chromatin and the maturating mRNA, with a general impact on splicing decisions.
Keywords :
Alternative splicing
Epigenetics
https://hal-pasteur.archives-ouvertes.fr/pasteur-02871948
Contributor : Violaine Saint-André <>
Submitted on : Wednesday, June 17, 2020 - 1:39:54 PM
Last modification on : Thursday, December 10, 2020 - 3:46:03 PM
Contributor : Violaine Saint-André <>
Submitted on : Wednesday, June 17, 2020 - 1:39:54 PM
Last modification on : Thursday, December 10, 2020 - 3:46:03 PM
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