Skip to Main content Skip to Navigation
Journal articles

The route of infection with Leptospira interrogans serovar Copenhageni affects the kinetics of bacterial dissemination and kidney colonization

Abstract : The goal of this study was to characterize how natural routes of infection affect the kinet- ics of pathogenic Leptospira dissemination to blood and kidney. C3H/HeJ mice were sub- lethally infected with L. interrogans serovar Copenhageni FioCruz L1-130 (Leptospira) through exposure of a dermis wound and through oral and nasal mucosa, in comparison to uninfected mice and to mice infected via standard intraperitoneal inoculation. In striking contrast to oral mucosa inoculation, transdermal and nasal mucosa infections led to weight loss, renal colonization and inflammation, as previously observed for conjunctival and intraperitoneal infections. However, the timing at which Leptospira gained access to blood, as well as Leptospira’ colonization of the kidney and shedding in urine, differed from intraperitoneal infection. Furthermore, a comparative analysis of transcription of pro- inflammatory mediators in kidney and total immunoglobulin isotyping in serum from infected mice, showed increased innate immune response markers (KC, MIP-2, TNF-α) and lower Th1 associated IFN-γ in kidney, as well as lower Th1 associated IgG2a in mice infected through the nasal mucosa as compared to intraperitoneal infection. We conclude that the route of infection affects the timing at which Leptospira gains access to blood for dissemination, as well as the dynamics of colonization and inflammation of the kidney.
Complete list of metadatas

Cited literature [31 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-02871134
Contributor : Catherine Werts <>
Submitted on : Wednesday, June 17, 2020 - 9:47:41 AM
Last modification on : Thursday, June 18, 2020 - 3:03:42 AM

File

journal.pntd.0007950 Nair 2020...
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Nisha Nair, Mariana Soares Guedes, Catherine Werts, Maria Gomes-Solecki. The route of infection with Leptospira interrogans serovar Copenhageni affects the kinetics of bacterial dissemination and kidney colonization. PLoS Neglected Tropical Diseases, Public Library of Science, 2020, 14 (1), pp.e0007950. ⟨10.1371/journal.pntd.0007950⟩. ⟨pasteur-02871134⟩

Share

Metrics

Record views

31

Files downloads

67