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Ethnicity-Specific Skeletal Muscle Transcriptional Signatures and Their Relevance to Insulin Resistance in Singapore

Amelia Li Min Tan 1, 2 Chee Fan Tan 3, 4 Jin Fang Chai 5 Chin Meng Khoo 2, 1, 6 Melvin Khee-Shing Leow 2, 7, 8, 9 Eric Yin Hao Khoo 1, 6 Suresh Anand Sadananthan 7 S Sendhil Velan 7, 10 Kavita Venkataraman 5 Yap Seng Chong 7, 1 Yung Seng Lee 7, 1, 11 Xueling Sim 5 Walter Stünkel 12 Mei Hui Liu 13 E Shyong Tai 1, 2, 6 Amelia Li 13 Min Tan 13 Sarah R Langley 2, 14 Fan Chee 4 Jin Fang Chai 13 Chin Meng Khoo 13 Melvin Khee 2 Shing Leow 2 Eric Yin 13 Hao Khoo 13 Aida Moreno-Moral 2 Michal Pravenec 15 Maxime Rotival 16 Enrico Petretto 17 
Abstract : Context: Insulin resistance (IR) and obesity differ among ethnic groups in Singapore, with the Malays more obese yet less IR than Asian-Indians. However, the molecular basis underlying these differences is not clear. Objective: As the skeletal muscle (SM) is metabolically relevant to IR, we investigated molecular pathways in SM that are associated with ethnic differences in IR, obesity, and related traits. Design, setting, and main outcome measures: We integrated transcriptomic, genomic, and phenotypic analyses in 156 healthy subjects representing three major ethnicities in the Singapore Adult Metabolism Study. Patients: This study contains Chinese (n = 63), Malay (n = 51), and Asian-Indian (n = 42) men, aged 21 to 40 years, without systemic diseases. Results: We found remarkable diversity in the SM transcriptome among the three ethnicities, with >8000 differentially expressed genes (40% of all genes expressed in SM). Comparison with blood transcriptome from a separate Singaporean cohort showed that >95% of SM expression differences among ethnicities were unique to SM. We identified a network of 46 genes that were specifically downregulated in Malays, suggesting dysregulation of components of cellular respiration in SM of Malay individuals. We also report 28 differentially expressed gene clusters, four of which were also enriched for genes that were found in genome-wide association studies of metabolic traits and disease and correlated with variation in IR, obesity, and related traits. Conclusion: We identified extensive gene-expression changes in SM among the three Singaporean ethnicities and report specific genes and molecular pathways that might underpin and explain the differences in IR among these ethnic groups. Trial registration: NCT00988819.
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Submitted on : Monday, June 15, 2020 - 5:52:45 PM
Last modification on : Thursday, April 7, 2022 - 10:10:38 AM

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Amelia Li Min Tan, Chee Fan Tan, Jin Fang Chai, Chin Meng Khoo, Melvin Khee-Shing Leow, et al.. Ethnicity-Specific Skeletal Muscle Transcriptional Signatures and Their Relevance to Insulin Resistance in Singapore. Journal of Clinical Endocrinology and Metabolism, 2019, 104 (2), pp.465-486. ⟨10.1210/jc.2018-00309⟩. ⟨pasteur-02869029⟩



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