, Fast SYBR-Green master mix (BIORAD, #170-8880AP) was used for the analysis of gene expression using the BIORAD CFX RT-PCR system. The primers used in the experiment are listed the in Supplementary Table 2. 18S was used to normalize the relative gene expression and the 2 ???Ct method was used to measure the fold change. Western blotting. Protein extracts were isolated from heart tissue and cells using RIPA buffer (Thermofischer, #89900) supplemented with protease (Sigma Aldrich, #11836170001) and phosphatase inhibitors cocktails (ROCHE, #PHOSS-RO). Nuclear and cytoplasmic extracts were obtained using NE-PER kit (Pierce, #78833) according to the manufacturer's instructions. Co-immunoprecipitation was performed with the cell lysates subjected to different treatment conditions with Pierce Direct Magnetic IP/CO-IP kit (Pierce, #88828) according to the manufacturer's protocol. Immunoprecipitates were washed from conjugated beads and boiled in 5× SDS-PAGE buffer for further WB analysis, :5000) and anti-GAPDH (Abcam, #ab8245, 1:5000). Anti-Lamin A/C (Abcam, #ab8984, 1:5000) and anti-PARP (Abcam, #ab6079,1:5000) were used as nuclear controls. Blots were visualized by labeling with anti-Rabbit HRP (Bethyl Laboratories, #A120-101P, 1:5000 or Thermo Fisher # 101023, 1:1000) and anti-Mouse HRP, vol.3102
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