Skip to Main content Skip to Navigation
Journal articles

Large-scale Exploration of Growth Inhibition Caused by Overexpression of Genomic Fragments in Saccharomyces cerevisiae

Abstract : We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.
Document type :
Journal articles
Complete list of metadatas

Cited literature [69 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-02868108
Contributor : Romain Koszul <>
Submitted on : Monday, June 15, 2020 - 12:14:17 PM
Last modification on : Wednesday, August 19, 2020 - 11:58:40 AM

File

document.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Jeanne Boyer, Gwenaël Badis, Cécile Fairhead, Emmanuel Talla, Florence Hantraye, et al.. Large-scale Exploration of Growth Inhibition Caused by Overexpression of Genomic Fragments in Saccharomyces cerevisiae. Genome Biology, BioMed Central, 2004, 5 (9), pp.R72. ⟨10.1186/gb-2004-5-9-r72⟩. ⟨pasteur-02868108⟩

Share

Metrics

Record views

27

Files downloads

57