Large-scale Exploration of Growth Inhibition Caused by Overexpression of Genomic Fragments in Saccharomyces cerevisiae

Jeanne Boyer (1) , Gwenaël Badis (1, 2) , Cécile Fairhead (1) , Emmanuel Talla (3, 1) , Florence Hantraye (2) , Emmanuelle Fabre (1) , Gilles Fischer (1) , Christophe Hennequin (4, 1) , Romain Koszul (1) , Ingrid Lafontaine (1) , Odile Ozier-Kalogeropoulos (1) , Miria Ricchetti (1, 5) , Guy-Franck Richard (6) , Agnès Thierry (6) , Bernard Dujon (6)

1 Génétique Moléculaire des Levures
2 Génétique des Interactions Macromoléculaires
3 LCB - Laboratoire de chimie bactérienne
4 Service de Parasitologie - Mycologie [CHU Saint-Antoine]
5 Génétique et Biochimie du Développement
6 Génétique Moléculaire des Levures

We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.

Domains: Life Sciences [q-bio]

Jeanne Boyer, Gwenaël Badis, Cécile Fairhead, Emmanuel Talla, Florence Hantraye, et al.. Large-scale Exploration of Growth Inhibition Caused by Overexpression of Genomic Fragments in Saccharomyces cerevisiae. Genome Biology, 2004, 5 (9), pp.R72. ⟨10.1186/gb-2004-5-9-r72⟩. ⟨pasteur-02868108⟩