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Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome

Abstract : Here, we report the successful design, construction, and characterization of a 770-kilobase synthetic yeast chromosome II (synII). Our study incorporates characterization at multiple levels-including phenomics, transcriptomics, proteomics, chromosome segregation, and replication analysis-to provide a thorough and comprehensive analysis of a synthetic chromosome. Our Trans-Omics analyses reveal a modest but potentially relevant pervasive up-regulation of translational machinery observed in synII, mainly caused by the deletion of 13 transfer RNAs. By both complementation assays and SCRaMbLE (synthetic chromosome rearrangement and modification by loxP-mediated evolution), we targeted and debugged the origin of a growth defect at 37°C in glycerol medium, which is related to misregulation of the high-osmolarity glycerol response. Despite the subtle differences, the synII strain shows highly consistent biological processes comparable to the native strain.
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Contributor : Romain Koszul Connect in order to contact the contributor
Submitted on : Monday, June 15, 2020 - 12:08:32 PM
Last modification on : Monday, May 9, 2022 - 11:58:08 AM

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Yue Shen, Yun Wang, Tai Chen, Feng Gao, Jianhui Gong, et al.. Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome. Science, 2017, 355 (6329), pp.eaaf4791. ⟨10.1126/science.aaf4791⟩. ⟨pasteur-02868099⟩



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