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Dynamic Processing of Displacement Loops during Recombinational DNA Repair

Abstract : Displacement loops (D-loops) are pivotal intermediates of homologous recombination (HR), a universal DNA double strand break (DSB) repair pathway. We developed a versatile assay for the physical detection of D-loops in vivo, which enabled studying the kinetics of their formation and defining the activities controlling their metabolism. Nascent D-loops are detected within 2 h of DSB formation and extended in a delayed fashion in a genetic system designed to preclude downstream repair steps. The majority of nascent D-loops are disrupted by two pathways: one supported by the Srs2 helicase and the other by the Mph1 helicase and the Sgs1-Top3-Rmi1 helicase-topoisomerase complex. Both pathways operate without significant overlap and are delineated by the Rad54 paralog Rdh54 in an ATPase-independent fashion. This study uncovers a layer of quality control of HR relying on nascent D-loop dynamics.
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Contributor : Romain Koszul <>
Submitted on : Monday, June 15, 2020 - 12:02:14 PM
Last modification on : Wednesday, June 17, 2020 - 3:27:11 AM

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Aurele Piazza, Shanaya Shital Shah, William Douglass Wright, Steven Gore, Romain Koszul, et al.. Dynamic Processing of Displacement Loops during Recombinational DNA Repair. Molecular Cell, Elsevier, 2019, 73 (6), pp.1255-1266.e4. ⟨10.1016/j.molcel.2019.01.005⟩. ⟨pasteur-02868091⟩

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