Trinucleotide repeat instability during double-strand break repair: from mechanisms to gene therapy - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Current Genetics Année : 2019

Trinucleotide repeat instability during double-strand break repair: from mechanisms to gene therapy

Résumé

Trinucleotide repeats are a particular class of microsatellites whose large expansions are responsible for at least two dozen human neurological and developmental disorders. Slippage of the two complementary DNA strands during replication, homologous recombination or DNA repair is generally accepted as a mechanism leading to repeat length changes, creating expansions and contractions of the repeat tract. The present review focuses on recent developments on double-strand break repair involving trinucleotide repeat tracts. Experimental evidences in model organisms show that gene conversion and break-induced replication may lead to large repeat tract expansions, while frequent contractions occur either by single-strand annealing between repeat ends or by gene conversion, triggering near-complete contraction of the repeat tract. In the second part of this review, different therapeutic approaches using highly specific single- or double-strand endonucleases targeted to trinucleotide repeat loci are compared. Relative efficacies and specificities of these nucleases will be discussed, as well as their potential strengths and weaknesses for possible future gene therapy of these dramatic disorders.
Fichier principal
Vignette du fichier
CUGE-D-18-00094_V4.pdf (2.05 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

pasteur-02864590 , version 1 (11-06-2020)

Identifiants

Citer

Valentine Mosbach, Lucie Poggi, Guy-Franck Richard. Trinucleotide repeat instability during double-strand break repair: from mechanisms to gene therapy. Current Genetics, 2019, 65, pp.17 - 28. ⟨10.1007/s00294-018-0865-1⟩. ⟨pasteur-02864590⟩
49 Consultations
432 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More