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Induction of type-IIA secretory phospholipase A2 in animal models of acute lung injury

Abstract : The aim of this study was to evaluate the presence of type-II secretory phospholipase A2 (sPLA2-IIA) in alveolar space and its possible role in the destruction of surfactant in three rat models of acute lung injury. Alveolar instillation of either lipopolysaccaride or live Pseudomonas aeruginosa resulted in a significant increase in lung oedema and in a decrease in static compliance of the respiratory system together with alveolar-neutrophil influx as compared with healthy control rats. The upregulation of messenger ribonucleic acid and sPLA2-IIA by the lung was evident. This was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in bacteria-instilled rats. A negative correlation between compliance and sPLA2-IIA activity in bronchoalveolar lavage fluid was shown. By contrast, during alpha naphthylthiourea-induced injury, neither alveolar-neutrophil influx nor increase in sPLA2-IIA activity was observed. Additional experiments in rats treated with a specific inhibitor of type-II secretory phospholipase A2 activity (3 acetamine-1-benzyl-2 ethylindolyl-5 oxy; propane phosphonic acid (LY311727)) demonstrated no improvement in physiological parameters despite a biochemical effect, suggesting that its activity is only one of the multiple factors involved in the pathophysiology of lung injury.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-02863076
Contributor : Yongzheng Wu <>
Submitted on : Tuesday, June 9, 2020 - 9:43:41 PM
Last modification on : Wednesday, October 14, 2020 - 3:40:33 AM

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H.L. Attalah, Yongzheng Wu, M. Alaoui-El-Azher, F. Thouron, K. Koumanov, et al.. Induction of type-IIA secretory phospholipase A2 in animal models of acute lung injury. European Respiratory Journal, European Respiratory Society, 2003, 21 (6), pp.1040-1045. ⟨10.1183/09031936.03.00093002⟩. ⟨pasteur-02863076⟩

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