Neisseria meningitidis pili induce type-IIA phospholipase A 2 expression in alveolar macrophages

Lhousseine Touqui (1) , Miguel Paya (1) , Francoise Thouron (1) , Annie Guiyoule (2) , Maria-Leticia Zarantonelli (2) , Dominique Leduc (1) , Yongzheng Wu (1) , Muhamed-Kheir Taha (2) , Jean-Michel Alonso (2)

1 Défense innée et inflammation
2 Neisseria

Induction of type-IIA secreted phospholipase A2 (sPLA2-IIA) expression by bacterial components other than lipopolysaccharide has not been previously investigated. Here, we show that exposure of alveolar macrophages (AM) to Neisseria meningitidis or its lipooligosaccharide (LOS) induced sPLA2-IIA synthesis. However, N. meningitidis mutant devoid of LOS did not abolish this effect. In addition, a pili-defective mutant exhibited significantly lower capacity to stimulate sPLA2-IIA synthesis than the wild-type strain. Moreover, pili isolated from a LOS-defective strain induced sPLA2-IIA expression and nuclear factor kappa B (NF-kappaB) activation. These data suggest that pili are potent inducers of sPLA2-IIA expression by AM, through a NF-kappaB-dependent process.

Domaines: Immunité innée; Bactériologie; Maladies infectieuses

Lhousseine Touqui, Miguel Paya, Francoise Thouron, Annie Guiyoule, Maria-Leticia Zarantonelli, et al.. Neisseria meningitidis pili induce type-IIA phospholipase A 2 expression in alveolar macrophages. FEBS Letters, 2005, 579 (22), pp.4923-4927. ⟨10.1016/j.febslet.2005.06.067⟩. ⟨pasteur-02863049⟩