A ribosome-bound quality control complex triggers degradation of nascent peptides and signals translation stress, Cell, vol.151, pp.1042-1054, 2012. ,
Ribosome-associated protein quality control, Nat Struct Mol Biol, vol.23, pp.7-15, 2016. ,
ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor, Cell, vol.121, pp.1071-1083, 2005. ,
Failure of RQC machinery causes protein aggregation and proteotoxic stress, Nature, vol.531, pp.191-195, 2016. ,
Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ, Mol Cell Proteomics, vol.13, pp.2513-2526, 2014. ,
Rkr1/Ltn1 ubiquitin ligasemediated degradation of translationally stalled endoplasmic reticulum proteins, J Biol Chem, vol.290, pp.18454-18466, 2015. ,
Cdc48-associated complex bound to 60S particles is required for the clearance of aberrant translation products, Proc Natl Acad Sci, vol.110, pp.5046-5051, 2013. ,
Rqc1 and Ltn1 prevent c-terminal alanine-threonine tail (CAT-tail)-induced protein aggregation by efficient recruitment of Cdc48 on stalled 60S subunits, J Biol Chem, vol.291, pp.12245-12253, 2016. ,
Nascent peptide-dependent translation arrest leads to Not4p-mediated protein degradation by the proteasome, J Biol Chem, vol.284, pp.10343-10352, 2009. ,
, , 2016.
, Structure and function of the yeast listerin (Ltn1) conserved N-terminal domain in binding to stalled 60S ribosomal subunits, Proc Natl Acad Sci, vol.113, pp.4151-4160
Endonucleolytic cleavage of eukaryotic mRNAs with stalls in translation elongation, Nature, vol.440, pp.561-564, 2006. ,
A putative ubiquitin ligase required for efficient mRNA export differentially affects hnRNP transport, Curr Biol, vol.10, pp.687-696, 2000. ,
The ubiquitin-proteasome system of saccharomyces cerevisiae, Genetics, vol.192, pp.319-360, 2012. ,
An mRNA surveillance mechanism that eliminates transcripts lacking termination codons, Science, vol.295, pp.2258-2261, 2002. ,
Global analysis of protein expression in yeast, Nature, vol.425, pp.737-741, 2003. ,
Hect E3 ubiquitin ligase Tom1 controls Dia 2 degradation during the cell cycle, Mol Biol Cell, vol.23, pp.4203-4211, 2012. ,
The Hect domain E3 ligase Tom1 and the F-box protein Dia 2 control Cdc6 degradation in G1 phase, J Biol Chem, vol.287, pp.44212-44220, 2012. ,
Structural basis for translational surveillance by the large ribosomal subunitassociated protein quality control complex, Proc Natl Acad Sci, vol.111, pp.15981-15986, 2014. ,
Protein quality control systems associated with no-go and nonstop mRNA surveillance in yeast, Genes Cells, vol.19, pp.1-12, 2014. ,
A series of ubiquitin binding factors connects CDC48/p97 to substrate multiubiquitylation and proteasomal targeting, Cell, vol.120, pp.73-84, 2005. ,
The heat shock response: life on the verge of death, Mol Cell, vol.40, pp.253-266, 2010. ,
A generic protein purification method for protein complex characterization and proteome exploration, Nat Biotechnol, vol.17, pp.1030-1032, 1999. ,
Structure and assembly pathway of the ribosome quality control complex, Mol Cell, vol.57, pp.433-444, 2015. ,
Reconstitution of a minimal ribosome-associated ubiquitination pathway with purified factors, Mol Cell, vol.55, pp.880-890, 2014. ,
Briefly, proteins were precipitated with the methanol-chloroform method (Wessel and Flügge, 1984) and digested with trypsin, and peptide samples were analyzed by LC-MS/MS on an Orbitrap Velos. Protein identifications and comparative label-free quantification were performed using the MaxQuant suite (version 1.5.2.8), which includes the Andromeda search engine. Quantifications were done using the algorithm integrated into MaxQuant to calculate Label Free Quantification intensities, mM N-ethylmaleimide, 10 µm MG-132, 1 mM PMSF, and water with EDTA-free protease inhibitor cocktail from Roche). Lysates were et, 2013. ,
Role of a ribosome-associated E3 ubiquitin ligase in protein quality control, Nature, vol.467, pp.470-473, 2010. ,
Isolation of mammalian 26S proteasomes and p97/VCP complexes using the ubiquitin-like domain from HHR23B reveals novel proteasome-associated proteins, Biochemistry, vol.48, pp.2538-2549, 2009. ,
Regulated protein turnover: snapshots of the proteasome in action, Nat Rev Mol Cell Biol, vol.15, pp.122-133, 2014. ,
Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications, Yeast, vol.14, pp.115-132, 1998. ,
Dom34:Hbs1 plays a general role in quality-control systems by dissociation of a stalled ribosome at the 3? end of aberrant mRNA, Mol Cell, vol.46, pp.518-529, 2012. ,
Exosome-mediated recognition and degradation of mRNAs lacking a termination codon, Science, vol.295, pp.2262-2264, 2002. ,
Cdc48/p97 promotes degradation of aberrant nascent polypeptides bound to the ribosome, vol.2, p.308, 2013. ,
The ribosome quality control pathway can access nascent polypeptides stalled at the Sec 61 translocon, Mol Biol Cell, vol.26, pp.2168-2180, 2015. ,
A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids, Anal Biochem, vol.138, pp.141-143, 1984. ,
The Rqc2/ Tae2 subunit of the ribosome-associated quality control (RQC) complex marks ribosome-stalled nascent polypeptide chains for aggregation, vol.5, p.11794, 2016. ,
Listerin-dependent nascent protein ubiquitination relies on ribosome subunit dissociation, Mol Cell, vol.50, pp.637-648, 2013. ,
Protein synthesis. Rqc2p and 60S ribosomal subunits mediate mRNA-independent elongation of nascent chains, Science, vol.347, pp.75-78, 2015. ,
URL : https://hal.archives-ouvertes.fr/hal-02680163
Dom34:Hbs1 promotes subunit dissociation and peptidyl-tRNA drop-off to initiate no-go decay, Science, vol.330, pp.369-372, 2010. ,
Kinetic analysis reveals the ordered coupling of translation termination and ribosome recycling in yeast, Proc Natl Acad Sci, vol.108, pp.1392-1398, 2011. ,
Histone levels are regulated by phosphorylation and ubiquitylation-dependent proteolysis, Nat Cell Biol, vol.11, pp.925-933, 2009. ,
A conserved quality-control pathway that mediates degradation of unassembled ribosomal proteins, vol.5, p.19105, 2016. ,