Compatibility of 5-ethynyl-2′F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Organic & Biomolecular Chemistry Année : 2019

Compatibility of 5-ethynyl-2′F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers

Christian Leumann
  • Fonction : Auteur
  • PersonId : 1043074

Résumé

A modified nucleoside triphosphate bearing two modifications based on a 2'-deoxy-2'-fluoro-arabinofuranose sugar and a uracil nucleobase equipped with a C5-ethynyl moiety (5-ethynyl-2'F-ANA UTP) was synthesized. This nucleotide analog could enzymatically be incorporated into DNA oligonucleotides by primer extension and reverse transcribed to unmodified DNA. This nucleotide could be used in SELEX for the identification of high binding affinity and nuclease resistant aptamers.
Fichier principal
Vignette du fichier
FANAmanuscript_revised_deposit.pdf (764.28 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

pasteur-02860714 , version 1 (06-07-2020)

Licence

Paternité - Pas d'utilisation commerciale - Partage selon les Conditions Initiales

Identifiants

Citer

Fabienne Levi-Acobas, Adam Katolik, Pascal Röthlisberger, Thomas Cokelaer, Ivo Sarac, et al.. Compatibility of 5-ethynyl-2′F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers. Organic & Biomolecular Chemistry, 2019, 17 (35), pp.8083-8087. ⟨10.1039/C9OB01515A⟩. ⟨pasteur-02860714⟩
61 Consultations
82 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More