6Biomics (plateforme technologique) (Centre de Ressources et Recherches technologiques (C2RT), Département Génomes et Génétique -25-28 rue du Docteur Roux, 75724 Paris cedex 15 - France)
Abstract : A modified nucleoside triphosphate bearing two modifications based on a 2'-deoxy-2'-fluoro-arabinofuranose sugar and a uracil nucleobase equipped with a C5-ethynyl moiety (5-ethynyl-2'F-ANA UTP) was synthesized. This nucleotide analog could enzymatically be incorporated into DNA oligonucleotides by primer extension and reverse transcribed to unmodified DNA. This nucleotide could be used in SELEX for the identification of high binding affinity and nuclease resistant aptamers.
https://hal-pasteur.archives-ouvertes.fr/pasteur-02860714 Contributor : Marcel HollensteinConnect in order to contact the contributor Submitted on : Monday, July 6, 2020 - 11:42:14 AM Last modification on : Tuesday, April 12, 2022 - 4:28:03 PM Long-term archiving on: : Friday, September 25, 2020 - 1:36:28 PM
Fabienne Levi-Acobas, Adam Katolik, Pascal Röthlisberger, Thomas Cokelaer, Ivo Sarac, et al.. Compatibility of 5-ethynyl-2′F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers. Organic and Biomolecular Chemistry, Royal Society of Chemistry, 2019, 17 (35), pp.8083-8087. ⟨10.1039/C9OB01515A⟩. ⟨pasteur-02860714⟩