Compatibility of 5-ethynyl-2′F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers

Fabienne Levi-Acobas (1, 2) , Adam Katolik (3, 4) , Pascal Röthlisberger (1, 2) , Thomas Cokelaer (5, 6) , Ivo Sarac (1, 2) , Masad J Damha (4) , Christian Leumann (3) , Marcel Hollenstein (1, 2)

1 Chimie bioorganique des acides nucléiques - Bioorganic chemistry of nucleic acids
2 Département de Génomes et Génétique - Department of Genomes and Genetics
3 University of Bern
4 McGill University = Université McGill [Montréal, Canada]
5 Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB
6 Biomics (plateforme technologique)

A modified nucleoside triphosphate bearing two modifications based on a 2'-deoxy-2'-fluoro-arabinofuranose sugar and a uracil nucleobase equipped with a C5-ethynyl moiety (5-ethynyl-2'F-ANA UTP) was synthesized. This nucleotide analog could enzymatically be incorporated into DNA oligonucleotides by primer extension and reverse transcribed to unmodified DNA. This nucleotide could be used in SELEX for the identification of high binding affinity and nuclease resistant aptamers.

Domains: Chemical Sciences; Chemical Sciences; Organic chemistry

Fabienne Levi-Acobas, Adam Katolik, Pascal Röthlisberger, Thomas Cokelaer, Ivo Sarac, et al.. Compatibility of 5-ethynyl-2′F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers. Organic & Biomolecular Chemistry, 2019, 17 (35), pp.8083-8087. ⟨10.1039/C9OB01515A⟩. ⟨pasteur-02860714⟩