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Journal articles
Compatibility of 5-ethynyl-2′F-ANA UTP with in vitro selection for the generation of base-modified, nuclease resistant aptamers
Abstract : A modified nucleoside triphosphate bearing two modifications based on a 2'-deoxy-2'-fluoro-arabinofuranose sugar and a uracil nucleobase equipped with a C5-ethynyl moiety (5-ethynyl-2'F-ANA UTP) was synthesized. This nucleotide analog could enzymatically be incorporated into DNA oligonucleotides by primer extension and reverse transcribed to unmodified DNA. This nucleotide could be used in SELEX for the identification of high binding affinity and nuclease resistant aptamers.
Cited literature [67 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-02860714
Contributor : Marcel Hollenstein Connect in order to contact the contributor
Submitted on : Monday, July 6, 2020 - 11:42:14 AM
Last modification on : Thursday, December 2, 2021 - 3:18:02 PM
Long-term archiving on: : Friday, September 25, 2020 - 1:36:28 PM
Contributor : Marcel Hollenstein Connect in order to contact the contributor
Submitted on : Monday, July 6, 2020 - 11:42:14 AM
Last modification on : Thursday, December 2, 2021 - 3:18:02 PM
Long-term archiving on: : Friday, September 25, 2020 - 1:36:28 PM
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