, Pipette Barrier (Filter) tips (Certified Free for RNases, DNases, sterile) (Thermo Fisher Scientific, Invitrogen, catalog numbers: AM126-45 (10 ?l), p.0

, 2 ml and 1.5 ml tubes (Eppendorf, p.3810

, 2 ml hard tissue homogenizing tubes, containing 2.8 mm ceramic (zirconium oxide) beads CK28 (Bertin Instruments, catalog number: P000911-LYSK0-A, for Precellys tissue homogenizer

, Glass Pasteur Pipettes (Merck, catalog number: CLS7095D5X)

, Adult C57BL/6J and B6(Cg)-Spp1t m1Blh /J mice (8-12 weeks of age)

, Phosphate buffered saline (PBS BioPerformance Certified, pH 7.4, Sigma-Aldrich, catalog number, pp.5368-5378

. Mommaerts, Molecular Biology Grade (Millipore, Merck, catalog number: 444203-250ML (Calbiochem) or alternatively dithiothreitol (DTT) may replace ?-ME, 2015.

, RW1 (RNA wash buffer containing ethanol) (Qiagen, catalog number: 74134)

, RPE (RNA wash buffer) (Qiagen, catalog number: 74134)

. Rneasy-r-plus-mini and . Kit, , p.74134

, Alternatively the Qiagen RNeasy Kit not including the gDNA separation columns may be used (Qiagen, p.75154

P. A. Bates, Transmission of Leishmania metacyclic promastigotes by phlebotomine sand flies, Int J Parasitol, vol.37, issue.10, pp.1097-1106, 2007.

S. M. Christensen, A. T. Belew, N. M. El-sayed, W. L. Tafuri, F. T. Silveira et al., Host and parasite responses in human diffuse cutaneous leishmaniasis caused by L. amazonensis, PLoS Negl Trop Dis, vol.13, issue.3, p.7152, 2019.

D. F. Cortes, M. B. Carneiro, L. M. Santos, T. C. Souza, T. U. Maioli et al., Low and high-dose intradermal infection with Leishmania major and Leishmania amazonensis in C57BL/6 mice, Mem Inst Oswaldo Cruz, vol.105, issue.6, pp.736-745, 2010.