, Pipette Barrier (Filter) tips (Certified Free for RNases, DNases, sterile) (Thermo Fisher Scientific, Invitrogen, catalog numbers: AM126-45 (10 ?l), p.0
, 2 ml and 1.5 ml tubes (Eppendorf, p.3810
, 2 ml hard tissue homogenizing tubes, containing 2.8 mm ceramic (zirconium oxide) beads CK28 (Bertin Instruments, catalog number: P000911-LYSK0-A, for Precellys tissue homogenizer
, Glass Pasteur Pipettes (Merck, catalog number: CLS7095D5X)
, Adult C57BL/6J and B6(Cg)-Spp1t m1Blh /J mice (8-12 weeks of age)
, Phosphate buffered saline (PBS BioPerformance Certified, pH 7.4, Sigma-Aldrich, catalog number, pp.5368-5378
, Molecular Biology Grade (Millipore, Merck, catalog number: 444203-250ML (Calbiochem) or alternatively dithiothreitol (DTT) may replace ?-ME, 2015.
, RW1 (RNA wash buffer containing ethanol) (Qiagen, catalog number: 74134)
, RPE (RNA wash buffer) (Qiagen, catalog number: 74134)
, , p.74134
, Alternatively the Qiagen RNeasy Kit not including the gDNA separation columns may be used (Qiagen, p.75154
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Low and high-dose intradermal infection with Leishmania major and Leishmania amazonensis in C57BL/6 mice, Mem Inst Oswaldo Cruz, vol.105, issue.6, pp.736-745, 2010. ,