Skip to Main content Skip to Navigation
New interface
Journal articles

Influence of genetic polymorphism on transcriptional enhancer activity in the malaria vector Anopheles coluzzii

Abstract : Enhancers are cis-regulatory elements that control most of the developmental and spatial gene expression in eukaryotes. Genetic variation of enhancer sequences is known to influence phenotypes, but the effect of enhancer variation upon enhancer functional activity and downstream phenotypes has barely been examined in any species. In the African malaria vector, Anopheles coluzzii, we identified candidate enhancers in the proximity of genes relevant for immunity, insecticide resistance, and development. The candidate enhancers were functionally validated using luciferase reporter assays, and their activity was found to be essentially independent of their physical orientation, a typical property of enhancers. All of the enhancers segregated genetically polymorphic alleles, which displayed significantly different levels of functional activity. Deletion mutagenesis and functional testing revealed a fine structure of positive and negative regulatory elements that modulate activity of the enhancer core. Enhancer polymorphisms segregate in wild A. coluzzii populations in West Africa. Thus, enhancer variants that modify target gene expression leading to likely phenotypic consequences are frequent in nature. These results demonstrate the existence of naturally polymorphic A. coluzzii enhancers, which may help explain important differences between individuals or populations for malaria transmission efficiency and vector adaptation to the environment.
Document type :
Journal articles
Complete list of metadata

Cited literature [50 references]  Display  Hide  Download
Contributor : Adrien Pain Connect in order to contact the contributor
Submitted on : Monday, May 25, 2020 - 4:49:46 PM
Last modification on : Tuesday, April 12, 2022 - 4:28:03 PM


Influence of genetic polymorph...
Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License




Luisa Nardini, Inge Holm, Adrien Pain, Emmanuel Bischoff, Daryl Gohl, et al.. Influence of genetic polymorphism on transcriptional enhancer activity in the malaria vector Anopheles coluzzii. Scientific Reports, 2019, 9 (1), pp.15275. ⟨10.1038/s41598-019-51730-8⟩. ⟨pasteur-02619362⟩



Record views


Files downloads