Broad-Range Papillomavirus Transcriptome as a Biomarker of Papillomavirus-Associated Cervical High-Grade Cytology - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Journal of Molecular Diagnostics Année : 2019

Broad-Range Papillomavirus Transcriptome as a Biomarker of Papillomavirus-Associated Cervical High-Grade Cytology

André Nazac
  • Fonction : Auteur
  • PersonId : 953698
Henri Marret
  • Fonction : Auteur

Résumé

Human Papillomaviruses (HPV) are responsible for over 99% of cervical cancers. Molecular diagnostic tests based on the detection of viral DNA or RNA have low Positive Predictive Values (PPV) for the identification of cancer or precancerous lesions. Triage with the Papanicolaou test lacks sensitivity and even when combined with molecular detection of high-risk HPV results in a significant number of unnecessary colposcopies. We have developed a broad range detection test of HPV transcripts to take a snapshot of the transcriptome of 16 high-risk or putative high-risk HPV in cervical lesions (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68, 73, and 82). The purpose of this novel molecular assay is to detect and type HPV-positive samples and to determine a combination of HPV reads at certain specific viral spliced junctions that can better correlate with high-grade cytology, reflecting the presence of precancerous cells. In a proof-of-concept study conducted on 55 patients, starting from cervical smears, we have shown that (i) HPV RNA-Seq can detect papillomaviruses with performances comparable to a widely used HPV reference molecular diagnostic kit, and (ii) a combination of the number of sequencing reads at specific early vs late HPV transcripts can be used as a marker of high-grade cytology, with encouraging diagnostic performances as a triage test.
Fichier principal
Vignette du fichier
hpvrnaseq-manuscript.pdf (1.3 Mo) Télécharger le fichier
Figure1.jpg (3.8 Mo) Télécharger le fichier
Figure2.jpg (443.26 Ko) Télécharger le fichier
Figure3.jpg (438.3 Ko) Télécharger le fichier
SupplementalFigure1.jpg (1.33 Mo) Télécharger le fichier
SupplementalFigure2.jpg (787.96 Ko) Télécharger le fichier
SupplementalFigure3.jpg (2.43 Mo) Télécharger le fichier
SupplementalTableS1.xlsx (102.59 Ko) Télécharger le fichier
SupplementalTableS2.xlsx (27.81 Ko) Télécharger le fichier
SupplementalTableS3.xlsx (148.47 Ko) Télécharger le fichier
SupplementalTableS4.xlsx (64.03 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

pasteur-02613507 , version 1 (20-05-2020)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Philippe Pérot, Anne Biton, Jacques Marchetta, Anne-Gaelle Pourcelot, André Nazac, et al.. Broad-Range Papillomavirus Transcriptome as a Biomarker of Papillomavirus-Associated Cervical High-Grade Cytology. Journal of Molecular Diagnostics, 2019, 21 (5), pp.768-781. ⟨10.1016/j.jmoldx.2019.04.010⟩. ⟨pasteur-02613507⟩
87 Consultations
109 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More