A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods

R.E. Hartnell (1) , L. Stockley (1) , W. Keay (1) , J.-P. Rosec (2) , D. Hervio-Heath (3) , H. van den Berg (4) , F. Leoni (5) , D. Ottaviani (5) , U. Henigman (6) , S. Denayer (7) , B. Serbruyns , F. Georgsson , G. Krumova-Valcheva , E. Gyurova , C. Blanco , S. Copin (8) , E. Strauch , K. Wieczorek , M. Lopatek , A. Britova , G. Hardouin (9) , B. Lombard (8) , P. In'T Veld (10) , Alexandre Leclercq (11, 12) , C. Baker-Austin (1)

1 CEFAS - Centre for Environment, Fisheries and Aquaculture Science [Weymouth]
2 Laboratoires Ministère de l'Economie et des Finances [Montpellier]
3 IFREMER - Institut Français de Recherche pour l'Exploitation de la Mer
4 RIVM - National Institute for Public Health and the Environment [Bilthoven]
5 IZSUM - Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
6 Institute for food hygiene and bromatology [Ljubljana, Slovenia]
7 WIV-ISP - Institut Scientifique de Santé Publique [Belgique] - Scientific Institute of Public Health [Belgium]
8 ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail
9 AFNOR - Association Française de Normalisation
10 NVWA - Netherlands Food and Consumer Product Safety Authority
11 CNRL - Centre National de Référence Listeria - National Reference Center Listeria
12 CC-OMS / WHO-CC - Centre collaborateur de l'OMS Listeria / WHO Collaborating Centre Listeria

Globally, vibrios represent an important and well-established group of bacterial foodborne pathogens. The European Commission (EC) mandated the Comite de European Normalisation (CEN) to undertake work to provide validation data for 15 methods in microbiology to support EC legislation. As part of this mandated work programme, merging of ISO/TS 21872-1:2007, which specifies a horizontal method for the detection of V. parahaemolyticus and V. cholerae, and ISO/TS 21872-2:2007, a similar horizontal method for the detection of potentially pathogenic vibrios other than V. cholerae and V. parahaemolyticus was proposed. Both parts of ISO/TS 21872 utilized classical culture-based isolation techniques coupled with biochemical confirmation steps. The work also considered simplification of the biochemical confirmation steps. In addition, because of advances in molecular based methods for identification of human pa-thogenic Vibrio spp. classical and real-time PCR options were also included within the scope of the validation. These considerations formed the basis of a multi-laboratory validation study with the aim of improving the precision of this ISO technical specification and providing a single ISO standard method to enable detection of these important foodborne Vibrio spp.. To achieve this aim, an international validation study involving 13 laboratories from 9 countries in Europe was conducted in 2013. The results of this validation have enabled integration of the two existing technical specifications targeting the detection of the major foodborne Vibrio spp., simplification of the suite of recommended biochemical identification tests and the introduction of molecular procedures that provide both species level identification and discrimination of putatively pathogenic strains of V. parahaemolyticus by the determination of the presence of theromostable direct and direct related haemolysins. The method performance characteristics generated in this have been included in revised international standard, ISO 21872:

Domaines: Sciences du Vivant [q-bio]; Microbiologie et Parasitologie; Bactériologie

R.E. Hartnell, L. Stockley, W. Keay, J.-P. Rosec, D. Hervio-Heath, et al.. A pan-European ring trial to validate an International Standard for detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus in seafoods. International Journal of Food Microbiology, 2019, 288, pp.58-65. ⟨10.1016/j.ijfoodmicro.2018.02.008⟩. ⟨pasteur-02586177⟩