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Journal Articles European Journal of Immunology Year : 2019

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

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Andreas Acs
  • Function : Author
Dieter Adam
  • Function : Author
Sabine Adam‐klages
  • Function : Author
William Agace
Nima Aghaeepour
  • Function : Author
Larissa Nogueira Almeida
  • Function : Author
Giorgia Alvisi
  • Function : Author
Graham Anderson
  • Function : Author
Immanuel Andrä
  • Function : Author
Francesco Annunziato
  • Function : Author
  • PersonId : 934993
Achille Anselmo
  • Function : Author
Sudipto Bari
  • Function : Author
Joana Barros‐martins
  • Function : Author
Wolfgang Bauer
  • Function : Author
Nicole Baumgarth
  • Function : Author
Dirk Baumjohann
  • Function : Author
Bianka Baying
  • Function : Author
Mary Bebawy
  • Function : Author
Burkhard Becher
Alfonso Blanco
  • Function : Author
Dominic Boardman
  • Function : Author
Christian Bogdan
  • Function : Author
Jessica Borger
  • Function : Author
Philip Boulais
  • Function : Author
Jolene Bradford
  • Function : Author
Dirk Brenner
  • Function : Author
Ryan Brinkman
  • Function : Author
Anna Brooks
  • Function : Author
Dirk Busch
  • Function : Author
Martin Büscher
  • Function : Author
Timothy Bushnell
  • Function : Author
Federica Calzetti
  • Function : Author
Garth Cameron
  • Function : Author
Ilenia Cammarata
  • Function : Author
Xuetao Cao
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Susanna Cardell
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Marco Cassatella
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Andrea Cavani
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Antonio Celada
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Pratip Chattopadhyay
  • Function : Author
Sue Chow
  • Function : Author
Eleni Christakou
  • Function : Author
Luka Čičin‐šain
  • Function : Author
Federico Colombo
Laura Cook
  • Function : Author
Anne Cooke
  • Function : Author
Andrea Cooper
  • Function : Author
Alexandra Corbett
Lorenzo Cosmi
  • Function : Author
Ana Cumano
  • Function : Author
Ljiljana Cvetkovic
  • Function : Author
van Duc Dang
  • Function : Author
Chantip Dang‐heine
  • Function : Author
Martin Davey
  • Function : Author
Derek Davies
  • Function : Author
Sara de Biasi
  • Function : Author
Genny del Zotto
Gelo Victoriano Dela Cruz
  • Function : Author
Michael Delacher
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Silvia Della Bella
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Günnur Deniz
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Mark Dessing
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James Di Santo
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Andreas Dolf
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Thomas Dörner
Michael Dustin
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Charles‐antoine Dutertre
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Friederike Ebner
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Sidonia Eckle
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Matthias Edinger
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Pascale Eede
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Götz R.A. Ehrhardt
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Marcus Eich
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Pablo Engel
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Anna Erdei
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Bart Everts
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Christine Falk
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Todd Fehniger
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Mar Felipo‐benavent
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Helen Ferry
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Markus Feuerer
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Andrew Filby
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Kata Filkor
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Marie Follo
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Irmgard Förster
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John Foster
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Gemma Foulds
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Britta Frehse
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Paul Frenette
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Stefan Frischbutter
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Wolfgang Fritzsche
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Anastasia Gangaev
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Natalio Garbi
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Brice Gaudilliere
Ricardo Gazzinelli
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Wilhelm Gerner
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Nicholas Gherardin
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Kamran Ghoreschi
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Lara Gibellini
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Florent Ginhoux
Keisuke Goda
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Dale Godfrey
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Christoph Goettlinger
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Jose González‐navajas
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Carl Goodyear
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Jane Grogan
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Daryl Grummitt
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Andreas Grützkau
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Claudia Haftmann
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Jonas Hahn
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Günter Hämmerling
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Leo Hansmann
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Christopher Harpur
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Susanne Hartmann
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Andrea Hauser
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Anja Hauser
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David Haviland
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David Hedley
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Daniela Hernández
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Guadalupe Herrera
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Christoph Hess
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Thomas Höfer
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Petra Hoffmann
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Kristin Hogquist
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Tristan Holland
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Rikard Holmdahl
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Pleun Hombrink
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Jessica Houston
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Bimba Hoyer
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Fang‐ping Huang
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Johanna Huber
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Christopher Hunter
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William Hwang
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Anna Iannone
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Florian Ingelfinger
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Sabine Ivison
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Hans‐martin Jäck
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Peter Jani
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Beatriz Jávega
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Toralf Kaiser
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Tomas Kalina
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Thomas Kamradt
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Steven Ketelaars
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Ahad Khalilnezhad
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Srijit Khan
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Jan Kisielow
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Paul Klenerman
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Jasmin Knopf
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Hui‐fern Koay
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Aaron Marshall
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Wlodzimierz Maslinski
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Anna Vittoria Mattioli
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Stephen Miller
Hans Minderman
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Jonni Moore
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Susann Müller
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Luis Enrique Muñoz
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Toshinori Nakayama
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Milena Nasi
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Lai Guan Ng
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Antonia Niedobitek
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José‐enrique O'Connor
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Aaron Ochel
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Anna Oja
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Annette Oxenius
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Raghavendra Palankar
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Isabel Panse
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Kovit Pattanapanyasat
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Livius Penter
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Pärt Peterson
Christian Peth
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Jordi Petriz
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A. Graham Pockley
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Henning Ulrich
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Edwin Pol
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Marc Veldhoen
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Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

Dates and versions

pasteur-02573762 , version 1 (14-05-2020)

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Andrea Cossarizza, Hyun‐dong Chang, Andreas Radbruch, Andreas Acs, Dieter Adam, et al.. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European Journal of Immunology, 2019, 49 (10), pp.1457-1973. ⟨10.1002/eji.201970107⟩. ⟨pasteur-02573762⟩
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