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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Andrea Cossarizza 1, * Hyun‐dong Chang 2, * Andreas Radbruch 2 Andreas Acs Dieter Adam Sabine Adam‐klages William Agace Nima Aghaeepour Mübeccel Akdis 3 Matthieu Allez 4, 5 Larissa Nogueira Almeida Giorgia Alvisi Graham Anderson Immanuel Andrä Francesco Annunziato 6 Achille Anselmo Petra Bacher 7 Cosima Baldari 8 Sudipto Bari Vincenzo Barnaba 9 Joana Barros‐martins Luca Battistini 10 Wolfgang Bauer Sabine Baumgart 11 Nicole Baumgarth Dirk Baumjohann Bianka Baying Mary Bebawy Burkhard Becher Wolfgang Beisker 12 Vladimir Benes 13 Rudi Beyaert 14 Alfonso Blanco Dominic Boardman Christian Bogdan Jessica Borger Giovanna Borsellino 15 Philip Boulais Jolene Bradford Dirk Brenner Ryan Brinkman Anna Brooks Dirk Busch Martin Büscher Timothy Bushnell Federica Calzetti Garth Cameron Ilenia Cammarata Xuetao Cao 16 Susanna Cardell Stefano Casola 17 Marco Cassatella Andrea Cavani Antonio Celada Lucienne Chatenoud 18 Pratip Chattopadhyay Sue Chow Eleni Christakou Luka Čičin‐šain Mario Clerici 19 Federico Colombo Laura Cook Anne Cooke Andrea Cooper Alexandra Corbett 20 Antonio Cosma 21 Lorenzo Cosmi Pierre Coulie 22 Ana Cumano 23 Ljiljana Cvetkovic van Duc Dang Chantip Dang‐heine Martin Davey Derek Davies Sara de Biasi Genny del Zotto Gelo Victoriano Dela Cruz Michael Delacher Silvia Della Bella Paolo Dellabona 24 Günnur Deniz Mark Dessing James Di Santo 25 Andreas Diefenbach 26 Francesco Dieli 27 Andreas Dolf Thomas Dörner 28 Regine Dress 29 Diana Dudziak 30 Michael Dustin 31 Charles‐antoine Dutertre Friederike Ebner Sidonia Eckle Matthias Edinger Pascale Eede Götz R.A. Ehrhardt Marcus Eich Pablo Engel Britta Engelhardt 32 Anna Erdei Charlotte Esser 33 Bart Everts Maximilien Évrard 34 Christine Falk Todd Fehniger Mar Felipo‐benavent Helen Ferry Markus Feuerer Andrew Filby Kata Filkor Simon Fillatreau 35 Marie Follo Irmgard Förster John Foster Gemma Foulds Britta Frehse Paul Frenette Stefan Frischbutter Wolfgang Fritzsche David Galbraith 36 Anastasia Gangaev Natalio Garbi Brice Gaudilliere Ricardo Gazzinelli Jens Geginat 37 Wilhelm Gerner Nicholas Gherardin Kamran Ghoreschi Lara Gibellini Florent Ginhoux 38 Keisuke Goda Dale Godfrey Christoph Goettlinger Jose González‐navajas Carl Goodyear Andrea Gori 39 Jane Grogan Daryl Grummitt Andreas Grützkau 40 Claudia Haftmann 41 Jonas Hahn Hamida Hammad 14 Günter Hämmerling Leo Hansmann Göran Hansson 42 Christopher Harpur Susanne Hartmann Andrea Hauser Anja Hauser David Haviland David Hedley Daniela Hernández Guadalupe Herrera Martin Herrmann 43 Christoph Hess Thomas Höfer 41 Petra Hoffmann Kristin Hogquist Tristan Holland Thomas Höllt 44 Rikard Holmdahl 45 Pleun Hombrink Jessica Houston Bimba Hoyer Bo Huang 46 Fang‐ping Huang Johanna Huber Jochen Huehn 47 Michael Hundemer 48 Christopher Hunter William Hwang Anna Iannone Florian Ingelfinger Sabine Ivison Hans‐martin Jäck Peter Jani Beatriz Jávega Stipan Jonjic 49 Toralf Kaiser Tomas Kalina Thomas Kamradt Stefan Kaufmann 50 Baerbel Keller 51 Steven Ketelaars Ahad Khalilnezhad Srijit Khan Jan Kisielow Paul Klenerman 52 Jasmin Knopf Hui‐fern Koay Katja Kobow Jay Kolls Wan Ting Kong Manfred Kopf 53 Thomas Korn Katharina Kriegsmann Hendy Kristyanto Thomas Kroneis Andreas Krueger Jenny Kühne Christian Kukat Désirée Kunkel 54 Heike Kunze‐schumacher Tomohiro Kurosaki 55 Christian Kurts 56 Pia Kvistborg Immanuel Kwok Jonathan Landry Olivier Lantz 57 Paola Lanuti Francesca Larosa Agnès Lehuen 58 Salomé Leibundgut‐landmann Michael Leipold Leslie Y.T. Leung Megan Levings 59 Andreia Lino Francesco Liotta Virginia Litwin Yanling Liu 60 Hans‐gustaf Ljunggren Michael Lohoff 61 Giovanna Lombardi 62 Lilly Lopez Miguel López‐botet Amy Lovett‐racke Erik Lubberts 63 Hervé Luche 64 Burkhard Ludewig 65 Enrico Lugli 66 Sebastian Lunemann 67 Holden Maecker Laura Maggi 68 Orla Maguire Florian Mair Kerstin Mair Alberto Mantovani 69 Rudolf Manz Aaron Marshall Alicia Martínez‐romero Gloria Martrus 67 Ivana Marventano 70 Wlodzimierz Maslinski Giuseppe Matarese Anna Vittoria Mattioli Christian Maueröder Alessio Mazzoni James Mccluskey 20 Mairi Mcgrath 71 Helen Mcguire Iain Mcinnes Henrik Mei 72 Fritz Melchers Susanne Melzer Dirk Mielenz Stephen Miller Kingston H.G. Mills 73 Hans Minderman Jenny Mjösberg 74 Jonni Moore Barry Moran Lorenzo Moretta 75 Tim Mosmann Susann Müller 76 Gabriele Multhoff 77 Luis Enrique Muñoz Christian Münz 78 Toshinori Nakayama Milena Nasi Katrin Neumann 79 Lai Guan Ng Antonia Niedobitek Sussan Nourshargh 80 Gabriel Nùñez 81 José‐enrique O'Connor Aaron Ochel Anna Oja Diana Ordonez Alberto Orfao 82 Eva Orlowski‐oliver Wenjun Ouyang Annette Oxenius Raghavendra Palankar Isabel Panse 41 Kovit Pattanapanyasat Malte Paulsen Dinko Pavlinic Livius Penter Pärt Peterson 83 Christian Peth Jordi Petriz Federica Piancone Winfried Pickl Silvia Piconese 9 Marcello Pinti 1 A. Graham Pockley Malgorzata Justyna Podolska Zhiyong Poon Katharina Pracht Immo Prinz 84 Carlo Pucillo Sally Quataert Linda Quatrini 85 Kylie Quinn 86 Helena Radbruch Tim Radstake Susann Rahmig Hans‐peter Rahn Bartek Rajwa 87 Gevitha Ravichandran Yotam Raz Jonathan Rebhahn Diether Recktenwald Dorothea Reimer Caetano Reis E Sousa Ester B.M. Remmerswaal Lisa Richter Laura Rico Andy Riddell Aja Rieger J. Paul Robinson 87 Chiara Romagnani 2 Anna Rubartelli Jürgen Ruland Armin Saalmüller Yvan Saeys 88 Takashi Saito 89 Shimon Sakaguchi 90 Francisco Sala‐de‐oyanguren Yvonne Samstag Sharon Sanderson Inga Sandrock Angela Santoni 91 Ramon Bellmàs Sanz Marina Saresella 70 Catherine Sautes‐fridman 92 Birgit Sawitzki 93 Linda Schadt Alexander Scheffold Hans Scherer Matthias Schiemann Frank Schildberg Esther Schimisky Andreas Schlitzer 38 Josephine Schlosser Stephan Schmid Steffen Schmitt 94 Kilian Schober Daniel Schraivogel Wolfgang Schuh 95 Thomas Schüler 96 Reiner Schulte Axel Ronald Schulz Sebastian Schulz 97 Cristiano Scottá Daniel Scott-Algara 98 David Sester T. Vincent Shankey Bruno Silva‐santos Anna Katharina Simon Katarzyna Sitnik Silvano Sozzani 99 Daniel Speiser 100 Josef Spidlen 101 Anders Stahlberg Alan Stall Natalie Stanley Regina Stark Christina Stehle Tobit Steinmetz Hannes Stockinger 102 Yousuke Takahama Kiyoshi Takeda Leonard Tan Attila Tarnok 103 Gisa Tiegs 104 Gergely Toldi Julia Tornack Elisabetta Traggiai Mohamed Trebak 105 Timothy I.M. Tree Joe Trotter John Trowsdale 106 Maria Tsoumakidou Henning Ulrich Sophia Urbanczyk Willem Veen 78 Maries Broek Edwin Pol Sofie van Gassen 107 Gert van Isterdael 108 René A.W. Lier Marc Veldhoen 109 Salvador Vento‐asturias Paulo Vieira 23 David Voehringer 110 Hans‐dieter Volk Anouk Borstel Konrad Volkmann Ari Waisman 111 Rachael Walker Paul Wallace 112 Sa Wang Xin Wang 113 Michael Ward 114 Kirsten Ward‐hartstonge Klaus Warnatz 51 Gary Warnes 115 Sarah Warth Claudia Waskow 116 James Watson Carsten Watzl Leonie Wegener Thomas Weisenburger Annika Wiedemann Jürgen Wienands Anneke Wilharm Robert John Wilkinson Gerald Willimsky James Wing Rieke Winkelmann Thomas Winkler Oliver Wirz 78 Alicia Wong Peter Wurst Jennie Yang Juhao Yang Maria Yazdanbakhsh Liping Yu 117 Alice Yue Hanlin Zhang 118 Yi Zhao 119 Susanne Maria Ziegler 67 Christina Zielinski Jakob Zimmermann 120 Arturo Zychlinsky 121, 98
* Corresponding author
37 Integrative Biology Program [Milano]
INGM - Istituto Nazionale Genetica Molecolare [Milano]
Abstract : These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
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Submitted on : Thursday, May 14, 2020 - 3:10:20 PM
Last modification on : Tuesday, October 20, 2020 - 6:06:01 PM

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Andrea Cossarizza, Hyun‐dong Chang, Andreas Radbruch, Andreas Acs, Dieter Adam, et al.. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European Journal of Immunology, Wiley-VCH Verlag, 2019, 49 (10), pp.1457-1973. ⟨10.1002/eji.201970107⟩. ⟨pasteur-02573762⟩

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