The spine neck filters membrane potentials, Proc. Natl. Acad. Sci. USA, vol.103, pp.17961-17966, 2006. ,
, Figure 7. The Effect of Filamin A Upregulation on Spine Maturation
, Representative IB for FLNA, myc, and actin in HEK293T cells under control or hypoxia (0.2% O 2 ) conditions or after transfection with mycFLNA D21-D23-WT (left). Also shown is densitometric quantification of FLNA
, Representative IB for myc and YFP after IP of myc in the presence of recombinant GST or GST-FLNA D21-D23 proteins from homogenates of HEK293T cells cotransfected with mycFLNA FL and PHD2 WT YFP. Left (input): total lysate control in co-transfected HEK293T cells. Densitometric quantification of the PHD2 WT YFP/ mycFLNA FL ratio is shown
Representative images of tdT + 14-DIV ctrl (C), mycFLNA D21-D23-WT (D), or mycFLNA D21-D23-3P/A (E) transfected MHNs. Also shown is quantification of protrusion density (F), protrusion length (G), and percentage of spines with a head (H) (n = 10 neurons, pp.324-731 ,
, PSD-95 (red) (I 0 , J 0 , and K 0 ) in 21-DIV tdT-transfected MHN (I-K) alone (I and I 0 ) or together with mycFLNA D21-D23-WT (J and J 0 ) or mycFLNA D21-D23-3P/A (K and K 0 ). The contours of the dendrites in (I), (J), and (K) are indicated in (I 0 ), -K 0 ) Immunostaining of vGlut (green)
, Quantification of the dendritic density of vGlut + /PSD-95 + co-clusters
, Representative images of 14-DIV MHNs co-transfected with tdT together with scrambled control shRNA (M), shPhd2 (N), shFlna (O), or both shPhd2 and shFlna (P)
, Quantification of protrusion density (Q), protrusion length (R), and percentage of spines with a head
, Data are mean ± SEM. *p < 0.05, ***p < 0.001 versus ctrl or scr
, , 2010.
, , 2010.
, , 1992.
A role for prolyl hydroxylase domain proteins in hippocampal synaptic plasticity, Hippocampus, vol.23, pp.861-872, 2013. ,
, , 2001.
Tissue oxygen tension and brain sensitivity to hypoxia, Respir. Physiol, vol.128, pp.263-276, 2001. ,
Molecular mechanisms of dendritic spine development and remodeling, Prog. Neurobiol, vol.75, pp.161-205, 2005. ,
Targeting of proteins of the striatin family to dendritic spines: role of the coiled-coil domain, Traffic, vol.7, pp.74-84, 2006.,
URL : https://hal.archives-ouvertes.fr/hal-00092658
Diminished activity-dependent brain-derived neurotrophic factor expression underlies cortical neuron microcircuit hypoconnectivity resulting from exposure to mutant huntingtin fragments, J. Pharmacol. Exp. Ther, vol.335, pp.13-22, 2010. ,
Actin in dendritic spines: connecting dynamics to function, J. Cell Biol, vol.189, pp.619-629, 2010. ,
HIFalpha targeted for VHLmediated destruction by proline hydroxylation: implications for O2 sensing, Science, vol.292, pp.464-468, 2001. ,
, Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation, Science, vol.292, pp.468-472
, , 2009.
, , 2008.
, Ubiquitin proteasome-mediated synaptic reorganization: a novel mechanism underlying rapid ischemic tolerance, J. Neurosci, vol.28, pp.50-59
The filamins: organizers of cell structure and function, Cell Adhes. Migr, vol.5, pp.160-169, 2011. ,
, , 2011.
, The actin binding domain of bI-spectrin regulates the morphological and functional dynamics of dendritic spines, PLoS ONE, vol.6, 16197.
Distinct regional and subcellular localization of the actin-binding, 2012. ,
, J. Comp. Neurol, vol.520, pp.3013-3034
, , 2012.
Translational control of mitochondrial energy production mediates neuron morphogenesis, Cell Metab, vol.16, pp.789-800, 2012. ,
Visualization and genetic manipulation of dendrites and spines in the mouse cerebral cortex and hippocampus using in utero electroporation, J. Vis. Exp, vol.26, p.4163, 2012. ,
Synaptic dynamics contribute to long-term single neuron response fluctuations, Front. Neural Circuits, vol.8, p.71, 2014. ,
MEKK4 signaling regulates filamin expression and neuronal migration, Neuron, vol.52, pp.789-801, 2006. ,
Grb4 and GIT1 transduce ephrinB reverse signals modulating spine morphogenesis and synapse formation, Nat. Neurosci, vol.10, pp.301-310, 2007. ,
Prolyl hydroxylase domain protein 2 (PHD2) binds a Pro-Xaa-Leu-Glu motif, linking it to the heat shock protein 90 pathway, J. Biol. Chem, vol.288, pp.9662-9674, 2013. ,
Filamins as integrators of cell mechanics and signalling, Nat. Rev. Mol. Cell Biol, vol.2, pp.138-145, 2001. ,
Regulation of dendritic spine motility and stability by Rac1 and Rho kinase: evidence for two forms of spine motility, Mol. Cell. Neurosci, vol.26, pp.429-440, 2004. ,
Identification of a novel protein (VBP-1) binding to the von Hippel-Lindau (VHL) tumor suppressor gene product, Biochim. Biophys. Acta, vol.56, pp.99-117, 1996. ,
Prolyl hydroxylase domain (PHD) 2 affects cell migration and F-actin formation via RhoA/rho-associated kinase-dependent cofilin phosphorylation, J. Biol. Chem, vol.285, pp.33756-33763, 2010. ,
Emerging novel functions of the oxygen-sensing prolyl hydroxylase domain enzymes, Trends Biochem. Sci, vol.38, pp.3-11, 2013. ,
Brefeldin A-inhibited guanine exchange factor 2 regulates filamin A phosphorylation and neuronal migration, J. Neurosci, vol.32, pp.12619-12629, 2012. ,
Filamin A regulates neuronal migration through brefeldin A-inhibited guanine exchange factor 2-dependent Arf1 activation, J. Neurosci, vol.33, pp.15735-15746, 2013. ,
MEK-ERK1/2-dependent FLNA overexpression promotes abnormal dendritic patterning in tuberous sclerosis independent of mTOR, Neuron, vol.84, pp.78-91, 2014. ,
Drosophila Ten-m and filamin affect motor neuron growth cone guidance, PLoS ONE, vol.6, 2011. ,
Hypoxia-induced and calpain-dependent cleavage of filamin A regulates the hypoxic response, Proc. Natl. Acad. Sci. USA, vol.111, pp.2560-2565, 2014. ,
Filamins in cell signaling, transcription and organ development, Trends Cell Biol, vol.20, pp.113-123, 2010. ,