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Journal articles
Lensless microscopy platform for single cell and tissue visualization
Ramona Corman
1, 2
Willem Boutu
2
Anna Campalans
3
Pablo Radicella
3
Joana Duarte
2
Maria Kholodtsova
2
Laure Bally-Cuif
4
Nicolas Dray
4
Fabrice Harms
1
Guillaume Dovillaire
1
Samuel Bucourt
1
Hamed Merdji
2
2
ATTO - Attophysique
IRAMIS - Institut Rayonnement Matière de Saclay, LIDyl - Laboratoire Interactions, Dynamiques et Lasers (ex SPAM)
Joana Duarte 2
Author
Maria N. Kholodtsova 2
Author
Laure Bally-Cuif 4
Author IdHAL : laure-bally-cuif IdRef : https://www.idref.fr/034709061
Nicolas Dray 4
Author IdHAL : nicolas-dray ORCID : https://orcid.org/0000-0002-2632-6004
1
Imagine Optic (France)
2
ATTO - Attophysique (France)
- IRAMIS - Institut Rayonnement Matière de Saclay (91191 Gif-sur-Yvette, France - France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DRF/IRAMIS (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
- LIDyl - Laboratoire Interactions, Dynamiques et Lasers (ex SPAM) (LIDYL, CEA, CNRS, Université Paris-Saclay, CEA Saclay 91190 Gif sur Yvette - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DRF/IRAMIS (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- CNRS - Centre National de la Recherche Scientifique : UMR9222 (France)
3
IRCM - Institut de Radiobiologie Cellulaire et Moléculaire (France)
- CEA - Commissariat à l'énergie atomique et aux énergies alternatives : DRF/IRCM (Centre de Saclay Centre de Grenoble Centre de Cadarache etc - France)
- Université Paris-Saclay (Espace Technologique, Bat. Discovery - RD 128 - 2e ét., 91190 Saint-Aubin - France)
4
Neurogénétique du Poisson zébré - Zebrafish Neurogenetics (Département de Biologie du Développement et Cellules Souches - 25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- Institut Pasteur [Paris] (25-28, rue du docteur Roux, 75724 Paris cedex 15 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR3738 (France)
Abstract : Today, 3D imaging techniques are emerging, not only as a new tool in early drug discovery but also for the development of potential therapeutics to treat disease. Particular efforts are directed towards in vivo physiology to avoid perturbing the system under study. Here, we assess non-invasive 3D lensless imaging and its impact on cell behavior and analysis. We test our concept on various bio-applications and present here the first results. The microscopy platform based on in-holography provides large fields of view images (several mm2 compared to several hundred µm2) with sub-micrometer spatial resolution. 3D image reconstructions are achieved using back propagation functions post-processing.
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Journal articles
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https://hal-pasteur.archives-ouvertes.fr/pasteur-02566019
Contributor : Nicolas Dray <>
Submitted on : Wednesday, May 6, 2020 - 5:54:51 PM
Last modification on : Wednesday, April 14, 2021 - 3:40:56 AM
Contributor : Nicolas Dray <>
Submitted on : Wednesday, May 6, 2020 - 5:54:51 PM
Last modification on : Wednesday, April 14, 2021 - 3:40:56 AM
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Identifiers
- HAL Id : pasteur-02566019, version 1
- DOI : 10.1364/BOE.380193
Collections
PASTEUR | CEA | CEA-UPSAY | IRAMIS-LIDYL | JACOB | CEA-DRF | HAL-PETACOM | CNRS | IRAMIS | GS-CHIMIE | GS-ENGINEERING
Citation
Ramona Corman, Willem Boutu, Anna Campalans, Pablo Radicella, Joana Duarte, et al.. Lensless microscopy platform for single cell and tissue visualization. Biomedical optics express, Optical Society of America - OSA Publishing, 2020, 11 (5), pp.2806. ⟨10.1364/BOE.380193⟩. ⟨pasteur-02566019⟩
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